Tissue availability is dependent on the collection schedule and prior sales. For tissue availability, contact the Office of Biological Resources and Resource Development Order Desk. Both Fischer (F) and Brown Norway (BN) animals and hybrids are available. Controls are caloric-restricted (CR) and ad libitum (AL).
Tissue Strains and Ages
Tissue is available from the following strains and ages:
F344 Rats: 4, 12, 18, 24, and 28 months
F344BN F1 Rats: 4, 18, 24, and 32 months
C57BL/6 Mice: 4, 12, 18, and 24 months
*CB6F1 (BALB/c x C57BL/6) Mice: 4, 18, 24, and 32
BALB/c Mice, CR and AL Controls: 8, 14, 20, and 24
*F344 Rats, CR and AL Controls: 4, 18, 24, and 28
*F344BN F1 Hybrid Rats, CR and AL Controls: 4, 12, 18, 24, and 34
* = males only
Animals are euthanized by carbon dioxide asphyxiation, and fresh, unfixed tissue and organs are flash frozen in LN 2 and stored at –80 degrees Celcius. Special arrangements can be made for NIA-funded investigators for fixation of tissues or collection of additional ages or tissues such as bone. For more information, please contact Dr. Nancy Nadon.
Collected Tissues
The following tissues will be collected from each animal:
- Serum (200 ul aliquots)
- Brain (whole or hemisphere)
- Cervical spinal cord
- Eyes (pair)
- Sciatic nerves (pair)
- Heart (whole, rinsed in phosphate-buffered saline)
- Lungs (whole)
- Liver (whole)
- Spleen (whole)
- Ovary or testes (pair)
- Prostate (male, whole)
- Muscle (thigh)
- Skin (shaved from flank, approximately 1 by 1 cm square)
- Kidneys (pair, whole)
- Adrenal gland (pair)
- White adipose tissue (subcutaneous)
- Brown adipose tissue
- Thymus (whole)
- Any tumors found
