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How do I design a primer?

There are several design criteria: GCG's prime program selects primers, and is also accessible through
GCG-Lite's web interface to prime.

Other web-accessible primer design programs are

Primer3 from the Whitehead Institute,
xprimer from U. Minnesota,
GeneFisher from U. Bielefeld.

Programs than run on Macs and PC's are MacVector for the Mac, GeneWorks for the Mac, OSP for Mac/PC/VAX/Sun/Xwindows, and primegen. Also check EBI's list of primer design programs.

More information about primer design can be found at:

Restriction Maps and PCR primer design, a course at NYU.
PCR primer analysis, a course from U. Kentucky.