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Xiphophorus Genetic Stock Center
Department of Chemistry and Biochemistry
Southwest Texas State University
419 Centennial Hall
San Marcos, Texas 78666-4616
URL: www.xiphophorus.org |
Principal
Investigator and Director
Ronald B. Walter, Ph.D.
512-245-0361; Fax: 512-245-1922
E-mail: RWalter@swt.edu
Codirector
Steven Kazianis, Ph.D.
512-245-0361; Fax: 512-245-1922
E-mail:
SK13@swt.edu
Contact
Leona Hazelwood
Manager
512-245-8469; Fax: 512-245-1922
E-mail:
LH24@swt.edu
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See contact listed above.
To provide the research community with animals from 65
pedigreed parental lines, representing 22 species,
in large numbers according to the specifications of
each investigator. The Xiphophorus Genetic Stock
Center (XGSC) will serve as a catalyst for continued
field studies aimed at identification and preservation
of new Xiphophorus species before their demise
due to habitat destruction. The stock center serves as
the bridge between production of genetically managed
research animals and their eventual dissemination and
use in research projects.
The stock center is continuing saturation of the
Xiphophorus gene map and development of expressed
sequence tag (EST) markers providing investigators with
comparative mapping data to approach complex scientific
questions. Ongoing efforts involve molecular characterization
of physical genetic markers (microsatellite and ESTs)
informative for gene mapping and genetic analyses in
several commonly used Xiphophorus interspecies crosses.
The stock center also is establishing technology that will
allow cryopreservation of Xiphophorus sperm samples
and thereby provide a preservation program where the XGSC
maintains the genetic heritage of all Xiphophorus strains
and species. The unique physiology of Xiphophorus sperm
has limited previous efforts at freezing of sperm for long-term
storage using standard protocols. In collaboration with Dr.
Terry Tiersch (LSU), recent advances in cryopreservation of
fish sperm are being studied to develop protocols allowing
long-term sperm storage and retrieval of active sperm samples.
Since its establishment over 70 years ago, the XGSC has provided
pedigreed fish from more than 60 genetic strains to scientists
and aquarists around the world. Scientists in more than 40
laboratories in the United States, Singapore, Canada, Mexico,
Japan, Columbia, and Germany work on Xiphophorus genetics
and depend on strains available from the XGSC.
Backcross hybrid tissues and nucleic acid panels are being
developed and will be available to investigators. Primer pair
sequences corresponding to genetic markers and assay
information for several interspecies crosses will be
made available to the research community.
In addition to supplying strains and providing consultation
on husbandry and genetic questions, the XGSC produces custom
interspecies hybrids (both first generation, F1 and
backcross hybrid generation BC1) for a variety of
projects, shipping hundreds of such fish each year. The XGSC
will assist research users to perform detailed genetic mapping
analyses on fish samples where a collaborating researcher has
scored a phenotype of interest.
Index Terms
Genetic stock center, pedigree management, inter-species
cross, gene map, complex traits.
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Zebrafish International Resource Center
5274 University of Oregon
Eugene, OR 97403-5274
URL: http://zfin.org/zf_info/stckctr/stckctr.html |
Principal
Investigator
Monte Westerfield, Ph.D.
541-346-4607; Fax: 541-346-4548
E-mail: monte@uoneuro.uoregon.edu
Contact
Pat Edwards
541-346-5222; Fax: 541-346-4548
E-mail:
edwards@uoneuro.uoregon.edu
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To provide a central repository for materials and
information about zebrafish research, as well as a stock
center for wild-type and mutant strains of zebrafish
(Danio rerio). Materials and zebrafish strains are
distributed to the research community. Pathology services
are provided for diseased fish. Standards and procedures
for maintaining healthy strains of zebrafish are being
developed and a manual for prevention, diagnosis, and
treatment of diseases affecting zebrafish is being
prepared.
Animals
Healthy stocks of zebrafish and frozen sperm are maintained
and distributed to the research community. Strains of
wild-type fish and lines carrying mutations and transgenes
are accepted from the research community, maintained, and
distributed on request. Embryos preserved in fixative at
various developmental stages are available from these lines
for histological, biochemical, or molecular biological
studies. Researchers are welcome to visit the resource
center to screen the collection for mutations of interest
or to learn procedures. Advice about zebrafish health is
provided. Diseased fish and tissue samples may be sent to
the resource center for pathology analysis. The center also
provides routine sentinel or quality control testing of
zebrafish from healthy laboratory colonies (e.g., to
fulfill IACUC health status monitoring).
Biological
Materials
Antibodies, gene probes, and other markers to analyze
wild-type and mutant stocks as well as zebrafish cell lines
are received, stored, and distributed.
Informatics
An online database, ZFIN
(http://zfin.org), of information
about zebrafish research is maintained, including
information about zebrafish genetics, genomics, and
development. Information about the resources and services
provided by the resource center is available, as well as a
listing of zebrafish researchers, laboratories, and
publications. ZFIN integrates information about the genetic
map and sequence being generated by the zebrafish genome
initiative and provides links to related information about
genes in other species-specific databases. Researchers can
use ZFIN to register and submit to the nomenclature
committee new names for mutant alleles and for genes
identified by molecular cloning.
Index Terms
Antibodies, cell lines, Danio rerio, database, DNA,
genetic map, genetics, genomics, mutations, stock center,
zebrafish.
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