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Department of Biology
Indiana University
1001 E. 3rd Street Bloomington, IN 47405-3700
URL: http://flystocks.bio.indiana.edu/
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Principal
Investigator and Contact
Kathleen A. Matthews, Ph.D.
812-855-5782; Fax: 812-855-2577
E-mail: matthewk@indiana.edu
Coinvestigators
Thomas C. Kaufman, Ph.D.
812-855-3033
E-mail: kaufman@bio.indiana.edu
Kevin R. Cook, Ph.D.
812-855-5782
E-mail: kcook@bio.indiana.edu
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The center collects, maintains, and distributes genetically defined strains of Drosophila melanogaster with
significant research value. Emphasis is placed on genetic
tools that are useful to a broad range of investigations.
These include basic stocks for genetic analysis such as
marker, balancer, mapping, and transposon-tagging strains;
mutant alleles of identified genes; defined sets of
deficiencies and a variety of other chromosomal
aberrations; engineered lines for somatic and germline
clonal analysis; GAL4 and UAS lines for targeted gene
expression; lacZ-reporter enhancer trap stocks with defined
expression patterns for marking tissues; and a collection
of mapped marked-transposon-tagged lethals.
Approximately 8,500 strains are currently available.
Up-to-date stock lists are available for searching,
browsing, or copying at the Internet site. An order form is
available at the same site. One small subculture is
provided of each requested stock. Larger quantities of
animals cannot be provided by the center. Requests may be
submitted by e-mail to
flystocks@bio.indiana.edu.
Stock center scientists are available to answer questions
about center stocks, use of the database, or general
questions about Drosophila genetics for researchers
new to the field.
New users of the collections must contact the center for a
Bloomington user number (BUN) before ordering stocks. User
fees are assessed at the beginning of each year for the
previous year's use. See the Internet site for current
fees. Index Terms
Drosophila, fly, genetic tools, mutants,
transposons. |
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Department of Genetics and Cell Biology
University of Minnesota
250 BioScience Center
1445 Gortner Avenue
St. Paul, MN 55108-1095
URL: http://biosci.cbs.umn.edu/CGC
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Principal
Investigator
Robert K. Herman, Ph.D.
Contact
Theresa L. Stiernagle
612-625-2265; Fax: 612-625-5754
E-mail: stier@biosci.cbs.umn.edu
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The Caenorhabditis Genetics Center (CGC) acquires,
maintains, and distributes genetic stocks and information
about stocks of the small free-living nematode
Caenorhabditis elegans for use by investigators
initiating or continuing research on this genetic model
organism. The CGC acquires and maintains a C.
elegans bibliography and publishes and distributes a
C. elegans research newsletter, The Worm
Breeder's Gazette (WBG), and a WBG subscriber
directory. The CGC acts as a clearinghouse for genetic
nomenclature and maintains the C. elegans genetic
map; these latter functions are managed as a subcontract
from the CGC by Dr. Jonathan Hodgkin at MRC Laboratory of
Molecular Biology, Cambridge CB2 2QH, England. The C.
elegans WWW server (http://elegans.swmed.edu),
which provides a single point of access to all C.
elegans information available on the Internet, is
supported by the CGC as a subcontract to the University of
Texas Southwestern Medical Center and Dr. Leon Avery.
Animals
The CGC's collection of more than 4,000 strains includes
one allele of each mapped gene, all available chromosome
rearrangements, and selected multiple-mutant stocks for
genetic mapping. The CGC also has stocks of nematode
species closely related to C. elegans. Requests for
strains should include a brief
statement of the research or training activity for which
the stocks are intended. Information about CGC stocks can
be obtained from the CGC Web site. The Worm
Breeder's Gazette
The WBG is distributed worldwide to approximately
500 subscribers who pay a small subscription fee. Each
volume consists of six issues distributed over 2 years.
Except for the last issue of each volume, which is devoted
to an updated genetic map, the WBG includes short
abstracts of preliminary findings, work in progress, and
other news about C. elegans. An electronic version
of the WBG is available at
http://elegans.swmed.edu/wli.
An updated WBG subscriber directory is available on
the CGC Web site. Nomenclature Information
Recommended guidelines and persons to contact for specific
questions about C. elegans genetic nomenclature can
be found at
http://elegans.swmed.edu/Genome/nomen.html.
CGC Bibliography
A periodically updated C. elegans bibliography,
including abstracts, is available on the CGC Web site or
from http://elegans.swmed.edu/.
Index Terms
C. elegans, nematode, nematode bibliography, stock
center, Worm Breeder's Gazette, worm nomenclature.
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Department of Developmental Biology and Genetics
Stanford University Medical School
279 Campus Drive
Stanford, CA 94305
URL: http://cmgm.stanford.edu/~kimlab/
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Principal
Investigator and Contact
Stuart K. Kim, Ph.D.
650-725-7671; Fax: 650-725-7739
E-mail: kim@cmgm.stanford.edu
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DNA microarrays can be used as powerful tools to determine
changes in gene expression patterns, such as occurs in
mutants, in transgenic animals expressing disease-related
genes, and in animals that have been treated with various
drugs. In 1998, the full genomic sequence of C.
elegans was finished. Thus, investigators can use DNA
microarrays to profile the transcriptional pattern of every
gene. For this purpose, the resource has generated DNA
microarrays containing essentially all of the genes in the
C. elegans genome (approximately 19,000 genes).
The main objective is to provide DNA microarray technology
to the C. elegans academic community by performing
DNA microarray experiments for C. elegans academic
laboratories.
DNA Microarray Experiments
The Microarray Resource will perform DNA microarray
experiments using RNA samples supplied by each laboratory.
Information on how to contact the center, grow worms, and
prepare RNA can be found at the URL above. The microarray
center can perform approximately 1,000 microarray
experiments per year, on a first come–first served
basis. Data from the microarray experiments are stored on a
Sun workstation at Stanford University and can be accessed
via the Internet. Data Analysis
The Microarray Resource will provide software for analysis
of the large amounts of gene expression data generated by
microarray experiments. Examples of software programs
include a program to generate lists of genes that are up-
or down-regulated in a particular experiment, a program to
cluster genes based on common gene expression patterns from
multiple microarray experiments, and a program to cluster
experiments based on common patterns of target genes.
Index Terms
C. elegans, chips, DNA microarray, gene clusters,
gene expression.
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Rosenstiel School of Marine and Atmospheric Science
University of Miami
4600 Rickenbacker Causeway
Miami, FL 33149
URL: www.rsmas.miami.edu/groups/sea-hares
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Principal
Investigator
Patrick J. Walsh, Ph.D.
E-mail: pwalsh@rsmas.miami.edu
Contact
Thomas R. Capo
305-361-4946; Fax: 305-361-4934
E-mail: tcapo@rsmas.miami.edu
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To provide research investigators with laboratory-reared
Aplysia californica of known age and standardized
environmental background, as well as their food source.
Primary goal is to optimize and standardize Aplysia
used by NIH investigators. This includes a health
monitoring program based on water quality testing and
animal bacteriological screening. Ancillary projects
include genetics studies to characterize the variation of
laboratory-reared animals in comparison to natural
populations, and neurophysiological studies of the nervous
system during development.
To Outside Investigators
Sibling animals of known ages and stages are available to
investigators throughout the year. On request
Aplysia and their food (red seaweed) are shipped via
Federal Express, overnight priority. Shipping and handling
costs are charged to the investigator. Special cohorts,
procedures, or manipulations of animal groups can be
arranged by contacting the persons named above.
Animals
The colony currently contains more than 10,000
laboratory-reared animals at various life stages and known
ages. All animals are produced from field-collected
broodstock, monitored for health, and randomly tested for
behavioral responses prior to shipping. Core Staff
Includes investigators carrying out studies of life
history, animal husbandry, disease prevention, genetic
variability, and neurophysiology. Guest Investigators
and Graduate Students
Guest investigators and graduate students interested in
studies of life history, culture, genetics, and
neurophysiology are encouraged to inquire. Selection will
be based on the relevance of the proposed study and the
availability of resources to meet individual needs.
Index Terms
Aplysia, gastropod, invertebrate animals, marine
animals, mollusk, neurophysiological model.
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The Marine Biomedical Institute
University of Texas Medical Branch
Galveston, TX 77555-1163
URL: www.nrcc.utmb.edu
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Principal
Investigator
Phillip G. Lee, Ph.D.
Contacts
John W. Forsythe, M.S.
409-772-3662; Fax: 409-772-6993
E-mail: john.forsythe@utmb.edu
Leigh Walsh
E-mail: lswalsh@utmb.edu
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To serve the biomedical research community's increased
needs for alternative invertebrate models by maintaining a
consistent year-round supply of live cephalopod mollusks.
These animals are suitable for a wide range of
physiological and molecular biological investigations.
Investigations are being conducted in the area of life
history related to improved animal husbandry. Further
studies focus on improving culture system design through
development of computer automation and innovative water
filtration technology. Current biomedical research on
cephalopods includes neurophysiology of the giant axon;
anatomy and neurophysiology of the equilibrium receptor
organ as a comparative model of the vestibular system of
invertebrates; chemoreception, basic nutrition, and protein
metabolism; cellular receptor function; and brain,
behavior, and learning.
The NRCC has built a computer-automated, environmentally
controlled, recirculating seawater laboratory for the
purpose of culturing cephalopods. The tank systems can be
used to conduct a variety of experiments never before
possible with cephalopods.
To Outside Investigators
Visiting researchers have access to dedicated facilities,
including wet and dry laboratory space, office space,
computer support and accommodations, as well as priority
access to all available live animal resources. Off-site
investigators can have live animals, dissected animal
tissues/body fluids from all life stages, and a variety of
molecular reagents (gene libraries and clones) delivered
year-round. Staff expertise and an extensive literature
library are available.
Animals/Materials
All life stages of the squid Sepioteuthis lessoniana
and the common cuttlefish Sepia officinalis are
available year-round from laboratory culture populations.
The squid Lolliguncula brevis is available
year-round from local waters; the squids Loligo
opalescens, Loligo pealeii, and Loligo
plei can be obtained seasonally on request. The
chambered nautilus, Nautilus pompilius, and
Octopus bimaculoides are available on request.
Animal costs vary by species and size. Any tissue or body
fluid from these animals can also be provided. Fees for
special services are negotiated on a case-by-case basis.
Index Terms
Animal resource, cuttlefish, invertebrate model,
neurophysiology, octopus, squid.
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The California Institute of Technology
Division of Biology 156-29
1201 East California Boulevard
Pasadena, CA 91125
URL: http://sea-urchin.caltech.edu/genome
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Principal
Investigator
Eric H. Davidson, Ph.D.
626-395-4937; Fax: 626-793-3047
E-mail: davidson@mirsky.caltech.edu
Co-investigator and Contact
R. Andrew Cameron, Ph.D.
626-395-8421; Fax: 626-793-3047
E-mail: acameron@caltech.edu
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The resource prepares and distributes high-density arrayed
filters (macroarrays) containing large genomic and cDNA
libraries from the sea urchin, Strongylocentrotus
purpuratus. The cDNA libraries represent every stage of
embryogenesis, several individual cell types, and various
adult tissues. The resource also grows and ships individual
library clones as identified and requested by users. The
resource also has available methodology for differential
subtractive hybridization on the macroarrays; measurement
of hybridization kinetics on microarrays to establish
useful parameters of sensitivity under diverse experimental
situations; development of probes for use on macroarrays
that represent any given regulatory state in the sea urchin
embryo; and completion of a custom-made, unique processing
system for quantitative differential analysis of macroarray
screens using complex probes. The majority of this
information is posted on the Web site listed above.
The collection of materials in the Sea Urchin Genome
Resource now includes 13 cDNA libraries and two genomic
libraries. The cDNA libraries are all about 100,000 clones
in size. They are stored in 384-well plates and
double-spotted to facilitate identification of positive
clones against hybridization background. Each nylon filter
contains 18,432 clones in duplicate. Thus, 100,000 clone
libraries occupy six filters. The two genomic libraries are
prepared in PACs and BACs, respectively. These genomic
libraries have also been carried through the macroarray
process. A sequence tag connector (STC) map of the genome
at a resolution of 10 kilobases has been prepared from the
BAC library clones. Searchable databases of these sequences
are available on the Web site, although all published
sequences are in Genbank. For example, a catalog of the
most frequent precise repeat families and simple sequence
repeats (SSR) is posted on the Web site. Lists of gene
matches from the STCs and some small EST projects are also
published there. Requests for macroarray filter sets can be
submitted by email to acameron@caltech.edu.
Questions concerning use of the resource can also be made
to this address. Filter sets are distributed for $500,
which also includes a reasonable number of subsequent clone
orders. Index Terms
Development, genome, sea urchin, Strongylocentrotus
purpuratus.
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