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Protocol Number:
93-I-0057
- Title:
Apheresis Procedures to Obtain Plasma or Leukocytes for In Vitro Studies
- Number:
93-I-0057
- Summary:
This study will collect blood plasma and white blood cells for laboratory research using a procedure called apheresis. Apheresis is a method of collecting larger quantities of certain blood components than can safely be collected through a simple blood draw.
Patients 7 years of age and older with a parasitic infection or condition associated with a parasitic infection (i.e., elevated levels of IgE antibodies or of a type of white cell called eosinophils) who are currently enrolled in a NIH clinical research protocol may be eligible for this study. Relatives of patients and normal healthy volunteers will also be enrolled. Candidates will have a medical history, physical examination and blood tests. Individuals weighing less than 25 kilograms (55 pounds) may not participate.
Participants will undergo one of the following two apheresis procedures:
- Automated pheresis - Whole blood is drawn through a needle placed in an arm vein and circulated through a cell separator machine. The plasma (liquid part of the blood) and white cells are extracted, and the red cells are re-infused into the donor through a needle in the other arm. The procedure takes 1 to 2 hours.
- Manual pheresis - Whole blood is drawn through a needle placed in an arm vein and circulated through the cell separator machine. The red blood cells are separated from the rest of the blood and returned to the donor through the same needle. Usually only one needle stick is required and the procedure takes from 30 to 45 minutes. This method is used only in individuals who weigh less than 35 kg (77 pounds).
- Sponsoring Institute:
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National Institute of Allergy and Infectious Diseases (NIAID)
- Recruitment Detail
- Type:
Active Accrual Of New Subjects
- Gender:
Male & Female
- Referral Letter Required:
No
- Population Exclusion(s):
None
- Eligibility Criteria:
INCLUSION CRITERIA:
Children over the age of 7 years who weigh at least 25 kg and would not require sedation for apheresis will be included.
EXCLUSION CRITERIA:
Patients will not undergo apheresis procedures if they have cardiovascular instability, severe anemia (Hct less than 30), inadequate venous access, severe coagulation disorders (PTT or PT greater than 1.5 normal), are pregnant, or have any other condition which the attending physician or Apheresis Unit staff considers a contraindication to the procedure.
The Hetastarch procedure will not be used in patients with hypertension or evidence of fluid retention.
- Special Instructions:
Currently Not Provided
- Keywords:
-
Plasmapheresis
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Lymphocytes
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Eosinophils
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Neutrophils
- Recruitment Keywords:
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None
- Conditions:
-
Leukopheresis
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Plasmapheresis
- Investigational Drug(s):
- None
- Investigational Device(s):
- None
- Contacts:
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Patient Recruitment and Public Liaison Office
Building 61 10 Cloister Court Bethesda, Maryland 20892-4754 Toll Free: 1-800-411-1222 TTY: 301-594-9774 (local),1-866-411-1010 (toll free) Fax: 301-480-9793 Electronic Mail:prpl@mail.cc.nih.gov
- Citations:
Not Provided
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Warren Grant Magnuson Clinical Center (CC) National Institutes of Health (NIH)
Bethesda, Maryland 20892. Last update: 10/19/2004
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