INCLUSION CRITERIA:
Normal Volunteers - ages 18-60:
Healthy without active infection or history of recurrent infections.
Normal renal function (creatinine less than or equal to 1.5 mg/dl; less than or equal to 1+ proteinuria); normal hepatic function (bilirubin less than or equal to 1.5 mg/dl); normal hematologic function (WBC greater than or equal to 3000/mm(3); granulocytes greater than or equal to 1500/mm(3); platelets greater than or equal to 175,000; hemoglobin greater than or equal to 12.5).
Normal female volunteers of childbearing potential may be entered if using effective contraception, and with a negative urine pregnancy test determined the day before beginning G-CSF administration.
Patients - ages 18-60:
Systemic mastocytosis patients must have mast cell hyperplasia compatible with a diagnosis of systemic mastocytosis; and be without active infection or history of recurrent infections.
Preserved renal function (creatinine less than or equal to 2mg/dl; less than or equal to 2+ proteinuria); preserved hepatic function (bilirubin less than or equal to 1.5 mg/dl); preserved hematologic function (WBC greater than or equal to 3000/mm(3), granulocytes greater than or equal to 1500/mm(3); platelets greater than or equal to 175,000; hemoglobin greater than or equal to 12.5).
Normal female volunteers of childbearing potential may be entered if using effective contraception, and with a negative urine pregnancy test determined the day before beginning G-CSF administration.
EXCLUSION CRITERIA:
Normal Volunteers:
Active bacterial, fungal or viral infections.
HIV+ testing.
Pregnancy or lactation.
History of autoimmune disease such as rheumatoid arthritis, vasculitis, pyoderma gangrenosum or similar disorder.
Use of any investigative drugs.
Systemic Mastocytosis Patients:
Active bacterial, fungal or viral infections.
HIV+ testing.
Patients taking any other growth factors, cytokines or investigative drugs.
Patients hemodynamically unstable (blood pressure systolic of lower than 105 or diastolic lower than 65).
Pregnancy or lactation.
History of autoimmune disease such as rheumatoid arthritis, vasculitis, pyoderma gangrenosum or similar disorder.
History of splenic rupture.