INCLUSION CRITERIA:
ASTHMATICS:
Patients will be between 18 and 65 years of age, male or female.
The diagnosis of asthma requires a history of intermittent, reversible expiratory flow limitation.
Patients will have either an abnormal methacholine challenge or evidence of ongoing reversible airway obstruction. An abnormal methacholine challenge will be defined as a decrease in FEV(1) of at least 20% following inhalation of less than or equal to 4 mg/ml of methacholine. Ongoing reversible airway obstruction will be defined as an improvement of at least 12% and 200 cc in either the FEV(1) or FVC following bronchodilator treatment.
Normal complete blood count, PT, PTT, and serum electrolytes, mineral and hepatic panels.
Normal EKG and chest radiograph without acute pulmonary infiltrates.
For women of childbearing potential, negative pregnancy test prior to study and willingness to adhere to reliable birth control methods during the study.
RESEARCH VOLUNTEERS:
Research volunteers will be between 18 and 65 years of age, male or female.
A negative inhalational methacholine challenge as defined by the absence of a 20% decrease in FEV(1), following inhalation of 16 mg/ml of methacholine.
Normal complete blood count, PT, PTT, and serum electrolytes, mineral and hepatic panels.
Negative hepatitis serology.
Normal EKG and chest radiograph.
For women of childbearing potential, negative pregnancy test within 2 weeks prior to study and willingness to adhere to reliable birth control methods during the study.
EXCLUSION CRITERIA:
ASTHMATICS:
Diagnosis of a pulmonary disorder other than asthma (e.g., chronic bronchitis, cystic fibrosis, bronchiectasis, HIV-related lymphocytic airway inflammation).
Respiratory tract infection or asthma exacerbation within 4 weeks of screening.
History of adverse reactions to lidocaine or other local anesthetics.
Allergy to methacholine.
Use of aspirin within 2 weeks of the bronchoscopic study or non-steroidal anti-inflammatory agents within 2 days of the bronchoscopic study.
History of cigarette smoking within the past 3 years.
History of drug or alcohol abuse.
Positive test for human immunodeficiency virus (to exclude patients with HIV-related lymphocytic airway inflammation).
Severe asthma as defined by the need for maintenance oral corticosteroid therapy or a FEV(1) less than 60 percent of predicted.
Active bronchospasm on physical examination.
History of lidocaine allergy.
History of prior cardiac disease.
RESEARCH VOLUNTEERS:
Same as the asthmatic exclusion criteria plus a history of asthma.
Warren Grant Magnuson Clinical Center (CC)