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Urinary Vitamin C Loss in Diabetic Subjects
This study is currently recruiting patients.
Sponsored by: | National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) |
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Information provided by: | Warren G Magnuson Clinical Center (CC) |
Purpose
The human body absorbs vitamin C from the intestine. Many different tissues store the vitamin but it is also lost in the urine. This study will examine whether patients with diabetes have low plasma vitamin C concentrations and lose more vitamin C in their urine than healthy people do. To find if this is the case, vitamin C concentrations in blood and urine will be measured. Certain proteins allow the body to absorb and store the vitamin. Variations in these proteins can be found by testing for genetic parameters called single nucleotide polymorphisms (SNPs), which are markers of genetic variation among people. We will test blood samples for specific SNPs related to vitamin C metabolism. This will show whether participants have genetic differences in the proteins that allow them to absorb and store vitamin C.
Patients 18 to 65 years of age who are either healthy or have diabetes, have no major illnesses other than diabetes, have no serious complications of the disease and whose blood pressure is well controlled may be eligible for this study.
Participants will choose to be studied either as outpatients or inpatients. They will undergo the following tests and procedures:
-Medical history
-Physical examination.
-Blood and urine tests for complete blood count, vitamin C, various chemistries, hepatitis B, hepatitis C, HIV, cotinine and other tests.
Participants will be asked to stop taking all vitamin preparations for 2 weeks before the study. Outpatients will provide a blood and urine sample after an overnight fast. The procedure will take a little over 1 hour. Inpatients will also provide a blood and urine sample, and afterward they will be hospitalized for 24 hours and placed on a diet free of vitamin C. They will be asked to eat and drink only food and beverages given to them during those 24 hours. A 24-hour urine sample will be collected for measurement of protein, creatinine, and vitamin C. Another blood sample will be collected on the morning of the second day, along with a sample of urine for vitamin C measurement.
Condition |
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Diabetes Mellitus |
MedlinePlus related topics: Diabetes
Study Type: Observational
Study Design: Screening
Official Title: Urinary Vitamin C Loss in Diabetics
Expected Total Enrollment: 150
Study start: October 24, 2003
Several studies have reported that diabetic subjects have lower plasma vitamin C concentrations than non-diabetic subjects. Although urinary vitamin C loss in diabetic subjects was reported to be increased in two studies, these are difficult to interpret due to lack of controlled vitamin C intake, inadequate sampling, lack of control subjects, or methodology uncertainties in vitamin C assay and sample processing. Consequently, it is unclear whether diabetic subjects truly have both low plasma and high urine vitamin C concentrations. We propose that low plasma vitamin C concentrations in diabetic subjects are due in part to inappropriate renal loss of vitamin C in these subjects but not in healthy controls. We will study vitamin C concentrations in patients with type 1 and type 2 diabetes and in matched healthy research subjects. Vitamin C concentrations in plasma, neutrophils (as a proxy for tissue concentrations) and in urine will be measured in outpatients. In those willing to be admitted to the Clinical Center, we will measure 24-hour urinary excretion of vitamin C while on a vitamin C free diet, and creatinine clearance, a measure of glomerular filtration rate. Single nucleotide polymorphisms (SNPs) will be determined in genomic DNA responsible for the two proteins mediating sodium-dependent vitamin C transport, SVCT1 and SVCT2. If low plasma and high urine vitamin C concentrations are found in diabetic subjects, further studies will be needed to explore mechanisms and to determine recommended dietary allowances for this patient population.
Eligibility
Genders Eligible for Study: Both
Accepts Healthy Volunteers
Criteria
Location and Contact Information
More Information
Publications
U.S. National Library of Medicine, Contact NLM Customer Service | ||||||||||||||
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