NSF Award Abstract - #0118961| NSF Award Abstract - #0118961 |
AWSFL008-DS3 |
| NSF Org | BES |
| Latest Amendment Date | July 9, 2003 |
| Award Number | 0118961 |
| Award Instrument | Continuing grant |
| Program Manager |
Fred G. Heineken BES DIV OF BIOENGINEERING & ENVIRON SYSTEMS ENG DIRECTORATE FOR ENGINEERING |
| Start Date | September 1, 2001 |
| Expires | August 31, 2005 (Estimated) |
| Expected Total Amount | $308965 (Estimated) |
| Investigator |
Mohammad M. Ataai ataai@engr.pitt.edu (Principal Investigator current) Richard R. Koepsel (Co-Principal Investigator current) |
| Sponsor |
U of Pittsburgh 350 Thackeray Hall Pittsburgh, PA 152602600 412/624-7400 |
| NSF Program | 1491 BIOTECHNOLOGY |
| Field Application | 0308000 Industrial Technology |
| Program Reference Code | 1063,9181,BIOT, |
The objective of the proposed research is the metabolic engineering of Bacillus subtilis for reduced acid formation and enhanced product yield. Preliminary work in both E. coli and B. subtilis showed that the addition of citrate allowed enhancements in growth and product formation. Acid formation was also substantially lowered. These improvements were tied to the level of pyruvate in the cell. Direct deletion of the pyk gene, NMR experiments, and flux modeling confirmed these results. The specific objectives of this research include: (1) improving the performance of B. subtilis which shows reduced growth with the pyk mutant, and (2) utilization of these results for the improvement of toxin production in Bacillus thuringiensis.
