Project
Title: CARBOHYDRATE ENGINEERING FOR GENERATING
SIALYLATED GLYCOPROTEINS IN INSECT CELLS
Investigator |
Institution |
Award Amount |
Award Time Period |
Michael J. Betenbaugh |
Johns Hopkins University |
|
FY1998-FY2000 |
Co Prinicipal
Investigators/Related Projects:
Donald L. Jarvis (University of Wyoming )
James Slavicek(USDA Forest Service)
Joseph Shiloach(National Institutes of Health)
Willie Vann(Food and Drug Administration)
Sponsored by: U.S. Dept. of Agriculture; National Institutes of Health; Food and
Drug Administration; National Science Foundation
Project Number:
NSF: 9814100
Brief Description of Project:
The inability of insect cells to generate glycoproteins similar in carbohydrate
composition to those of mammalian cells has limited the wider application of insect cells
as vehicles for the production of glycoproteins of mammalian origin. While mammalian cells
often produce glycoproteins containing complex oligosaccharides terminating in sialic
acid, insect cells typically generate glycoforms containing short truncated
oligosaccharides terminating in mannose or N-acetylglucosamine. The composition of these
covalently-attached carbohydrates, especially the presence of sialic acid, can
significantly affect a glycoprotein's structure, stability, biological activity, and in
vivo circulatory half-life. Consequently, the objective of this project is to manipulate
carbohydrate processing pathways in insect cells in such a way that these cells will
produce sialylated glycoproteins. This inter-disciplinary and inter- agency project will
involve participating scientists and engineers from Johns Hopkins University, the
University of Wyoming, the Food and Drug Administration (FDA), the National Institutes of
Health (NIH), and the Forest Service of the Department of Agriculture (USDA).
Project Starting Date: 08/98 (MM/YY)
Project Ending Date (Planned): 08/01 (MM/YY)
Research Area: |
Pathway Alteration |
Potential Field of Use: |
Basic Research, Pharmaceutical/Medical, Agriculture/Aquaculture |
Federal Contact Name:
FDA Contact: Willie Vann
Email: wvann@helix.nih.gov Phone: (301)496-2008
NIH Contact: Joseph Shiloach
Email: ljs@helix.nih.gov Phone: (301)496-9719
NSF Contact: Fred G. Heineken Email:
Fheineke@nsf.gov Phone: (703)306-1319
USDA Contact: James Slavicek
Email: jim.slavicek/ne_de@fs.fed.us Phone:
(740)368-0030
Last Revision: 02/22/00
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