Project
Title: STRATEGIES FOR
METABOLIC ENGINEERING OF ENVIRONMENTAL MICROORGANISMS -
APPLICATION TO DEGRADATION OF ORGANOPHOSPHATE CONTAMINANTS
| Investigator |
Institution |
Award Amount |
Award Time Period |
| Jay D. Keasling |
University of California-Berkeley |
|
FY1999-FY2001 |
Co Prinicipal
Investigators/Related Projects:
]No more investigators involved in this project.[
Sponsored by: U.S. Dept. of Defense; National Science
Foundation
Project Number:
NSF: 9814088
DOD: N000149910182
Brief
Description of Project:
This proposal seeks to develop the experimental and
theoretical
methods to introduce multiple, heterologous, biodegradation
pathways into a single organism and optimize the flux through
those pathways for the remediation of toxic or recalcitrant
organic contaminants. The project will focus the biodegradation
of parathion as a model compound since: (1) it has been widely
used as a pesticide; (2) it could potentially serve as a source
for carbon, phosphorus, and sulfur for cell growth; (3) no
single
organism has been isolated that can use parathion as a sole
carbon
and phosphorus source; and (4) it is similar in structure to a
number of other important environmental contaminants, such as
nerve agents and other pesticides. An engineered form of
Pseudomonas putida will be used as a model organism.
Supporting Publications:
Project
Starting Date: 01/99
Project
Ending Date (Planned): 12/01
| Research Area: |
Pathway Alteration |
| Potential Field of Use: |
Environmental |
Federal
Contact Name:
NSF Contact: Fred G. Heineken
E-Mail:
Fheineke@nsf.gov
Phone:(703)306-1319
DOD Contact: Harold Bright
E-Mail:
Brighth@onr.navy.mil
Phone:(703)696-4054
Last
Revision: 02/18/2000
|
