The objective of this project is to establish a
functional proteomics approach for the elucidation of the
important aromatic biosynthesis pathways of the extremely
thermoplilic sulfate-reducing archaeon, Archaeoglobus
fulgidus, whose genome was recently sequenced. In
particular, the Principal Investigators will design
techniques based on the coordinated use of liquid
chromatography/mass spectrometry (LC/MS), DNA microarray
technologies, and gene cloning and expression in E. coli for
efficient screening of enzyme activities and for gene
identification.
