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|New Mexico State University
|FY2000 to FY2001
Description of Project:
Metabolic engineering, the generation of organisms with
genetically engineered alterations in their metabolism is
one of the most rapidly growing fields of biotechnology and
one with huge prospective benefits to industry, agriculture
and the environment. This proposal is part of a project
(coordinated by Dr Andrew Hanson of U. Florida and involving
researchers at five universities) on engineering and
analyzing non-photosynthetic one-carbon metabolism in
plants. This cooperative agreement will bring together the
resources and experience in plant biochemistry and in vivo
NMR at NMSU with the facilities and expertise in advanced
NMR methods of analysing biomolecules of the Bioprocess
Engineering Group at NIST. The project involves analysis of
changes in metabolism and gene expression in transgenically
altered and naturally occuring mutant plant varieties of
tobacco and maize and aims at an understanding of the
regulation of an area of metabolism that is central to many
current and future engineering projects. The analysis
proposed here will use nuclear magnetic resonance (NMR)
spectroscopy to establish and apply metabolic profiling
methods, to analyze one-carbon (1-C) metabolic fluxes, and
to critically test models of 1-C metabolism.
Starting Date: 3/15/00
Ending Date (Planned): 3/14/01
Contact Name: NIST
Contact: Vincent L.
Last Revision: 10/31/00