<
 
 
 
 
>
hide
You are viewing a Web site, archived on 06:09:57 Oct 15, 2004. It is now a Federal record managed by the National Archives and Records Administration.
External links, forms, and search boxes may not function within this collection.

Metabolic Engineering Working Group

 
               
Print Page
Friendly Version
   
Home
Research
Events
Project Inventory
About MEWG






 

  Betenbaugh

 

Project Title: CARBOHYDRATE ENGINEERING FOR GENERATING SIALYLATED GLYCOPROTEINS IN INSECT CELLS

Investigator Institution Award Amount Award Time Period
Michael J. Betenbaugh Johns Hopkins University

$814706

FY1998-FY2000

Co Prinicipal Investigators/Related Projects:
Donald L. Jarvis (University of Wyoming )
James Slavicek(USDA Forest Service)
Joseph Shiloach(National Institutes of Health)
Willie Vann(Food and Drug Administration)

Sponsored by: U.S. Dept. of Agriculture; National Institutes of Health; Food and Drug Administration; National Science Foundation

Project Number:
NSF:   9814100

Brief Description of Project:

The inability of insect cells to generate glycoproteins similar in carbohydrate composition to those of mammalian cells has limited the wider application of insect cells as vehicles for the production of glycoproteins of mammalian origin. While mammalian cells often produce glycoproteins containing complex oligosaccharides terminating in sialic acid, insect cells typically generate glycoforms containing short truncated oligosaccharides terminating in mannose or N-acetylglucosamine. The composition of these covalently-attached carbohydrates, especially the presence of sialic acid, can significantly affect a glycoprotein's structure, stability, biological activity, and in vivo circulatory half-life. Consequently, the objective of this project is to manipulate carbohydrate processing pathways in insect cells in such a way that these cells will produce sialylated glycoproteins. This inter-disciplinary and inter- agency project will involve participating scientists and engineers from Johns Hopkins University, the University of Wyoming, the Food and Drug Administration (FDA), the National Institutes of Health (NIH), and the Forest Service of the Department of Agriculture (USDA).

Project Starting Date: 08/98 (MM/YY)

Project Ending Date (Planned): 08/01 (MM/YY)

Research Area: Pathway Alteration


Potential Field of Use: Basic Research, Pharmaceutical/Medical, Agriculture/Aquaculture



Federal Contact Name:
FDA Contact:    Willie Vann    Email: wvann@helix.nih.gov    Phone:   (301)496-2008
NIH Contact:    Joseph Shiloach    Email: ljs@helix.nih.gov    Phone:   (301)496-9719

NSF Contact: Fred G. Heineken    Email: Fheineke@nsf.gov    Phone:   (703)306-1319
USDA Contact:   James Slavicek   Email:   jim.slavicek/ne_de@fs.fed.us   Phone:   (740)368-0030

Last Revision: 02/22/00