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blue bulletWhat is dbEST?

dbEST (Nature Genetics 4:332-3;1993) is a division of GenBank that contains sequence data and other information on "single-pass" cDNA sequences, or Expressed Sequence Tags, from a number of organisms. A brief account of the history of human ESTs in GenBank is available (Trends Biochem. Sci. 20:295-6;1995). Also, consult the special "Genome Directory" issue of Nature (vol. 377, issue 6547S, 28 September 1995).

blue bulletOther ways to access dbEST

Other ways to access dbEST

blue bulletHow to submit data

How to submit data to dbEST

blue bulletInformation on the current release

Number of ESTs - dbEST summary by organism

 

blue bulletI.M.A.G.E. Consortium Clones

"Trace data" for sequences derived from I.M.A.G.E clones is available from the Washington University Genome Sequencing Center. Traces are retrieved using the identifier labelled "EST name" in the dbEST record (e.g. yb01a01.s1, corresponding to GenBank accession number T48601). Note that this identifier may also be found in the DEFINITION line of the GenBank Record:

DEFINITION yb01a01.s1 Homo sapiens cDNA clone 69864 3' similar to ...

Physical DNA clones from I.M.A.G.E. Consortium libraries are now available from a number of distributors . Clones may be ordered using the identifier labelled "Clone Id" in the dbEST record (e.g. 69864, corresponding to GenBank accession number T48601). As before, this identifier is also available in the DEFINITION line of the corresponding GenBank Record.

blue bulletUpdates
7/15/00 EST search method switched from IRX to Entrez. Use search box above instead of old search page.
4/26/99 Four new data fields were added to the EST table: POLYA, TAG_LIB, TAG_TISSUE, TAG_SEQ. The last 3 are used to indicate sequence tags for individual cDNA libraries which are included in a pooled library. For details see: How to submit data to dbEST
12/24/97 Contaminating Sequences Identified and Removed from dbEST
08/15/97 4800 EST Sequences Withdrawn


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Revised July 11, 2000