/reformat.html">
format conversion tool that converts between the formats that
Readseq uses. Paste your sequence into the input box, choose an
output format, and click on 'Submit Request'. The reformatted sequence
will appear in your web browser, where you can save it into a file on
your own machine. Transfer this file to helix using a file transfer
program such as Fetch or FTP.
Reformat cut off the end of my sequence.
One possible reason is that your input sequence was in one long
line without carriage returns. This can happen if you typed the
sequence in something like SimpleText, without carriage returns,
and then transferred it to helix. There are several ways around
this:
- Edit the file on helix,
and add a few carriage returns after every 50 bp or so. This is
reasonable as a one-time option; however, if you are going to be
doing this often you should use a less cumbersome method.
- When you type the sequence in your editor (the one on your
desktop machine), put carriage returns (i.e. hit the Enter key)
after every 50 or 80 characters.
- See if your editor (the one on your desktop machine) has an
option for saving the file with carriage returns.
- When you transfer the file, see if the file transfer program
has an option to insert carriage returns. For example, the
Fetch program has an option to transfer the file as 'Wrapped
Text'. This will break up the long lines, and Reformat will be
able to process the sequence successfully.
It is always worth checking the output from a reformatting
program! You don't need to examine the whole sequence, just
look at the beginning, the end and the length of the sequence.
How do I translate the coding regions of a Genbank entry?
Search for your protein by accession number or name in
Lookup.
In the 'Features:' field, type 'cds.
In the 'Form of output list' field, select 'Fragments'. (Use the
space bar on your keyboard to toggle options in this field).
Save the result in a file, which will contain the coding regi