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e As...' in your browser menu under File. Choose 'Text' as the format, and save the entry into a file on your desktop computer. This entry is in Genbank format, will need to be converted to GCG format for any GCG program. ou can use the helix program 'fmtseq' to convert it. Type 'fmtseq' at the helix prompt and follow instructions.

However the sequence is obtained, you will need to delete the extra bases on each end within seqed, and will have to keep track of the base numbering by yourself. Type 'seqed filename' to start up seqed. If you used Method 1, you will have two files to enter. Position your cursor at the end of the first sequence, hit Ctrl-D to get to the command prompt, and type 'Include filename2' to add the second sequence at the end of the first.

How can I search for promoter regions?

The GCG program Findpatterns can be used to find any pattern, and the GCG file tfsites.dat contains patterns from the Transcription Factor database. Sample session: 
helix 75% findpatterns -data=genmoredata:tfsites.dat

FindPatterns identifies sequences that contain short patterns like
GAATTC or YRYRYRYR.  You can define the patterns ambiguously and allow
mismatches. You can provide the patterns in a file or simply type them
in from the terminal

 FINDPATTERNS in what sequence(s) ? aw778747.gb_est46

  What should I call the output file (* aw778747.find *) ?

     aw778747.gb_est46 len:        410     ....................................
...............................................................................
...............................................................................
..................................................

 FINDPATTERNS in what sequence(s) ?
      
     Total finds:             45
    Total length:            410
 Total sequences:              1
        CPU time:          01.63

     Output file: aw778747.find
The file tfsites.dat is in the GCG directory genmoredata. You can copy this to your own directory using the 'fetch' program. e.g. 
helix% fetch tfsites.dat

Fetch copies GCG sequences or data files from the GCG database
into your directory or displays them on your terminal screen.

 tfsites.dat
You can then modify this file to retain only the sites of your interest.