Prepared by:
Tufts-New England Medical Center Evidence-based Practice Center
Boston, Massachusetts
Investigators
Harmon Jordan, Sc.D., Project Leader
Nirupa Matthan, Ph.D., Co-Project Leader
Mei Chung, M.P.H., Research Associate
Ethan Balk, M.D., M.P.H., Methodologist
Priscilla Chew, M.D., Research Associate
Bruce Kupelnick, B.A., Research Associate
Deirdre DeVine, M.Litt., Project Manager
Amy Lawrence, B.A., Research Assistant
Alice Lichtenstein, D.Sc., Primary Technical Expert
Joseph Lau, M.D., Principal Investigator
File Name Description Software Version File Size ________________________________________________________________________________________________ 01front.doc Microsoft Word® Document MS Word® 2002 87KB 14 pages Contents: Title Page, Preface, Acknowledgments, Technical Expert Panel, Structured Abstract, Table of Contents ________________________________________________________________________________________________ 02body.doc Microsoft Word® Document MS Word® 2002 1.6MB 74 pages Contents: Chapter 1. Introduction: Metabolism and Biological Effects of Essential Fatty Acids; Metabolic Pathways of Omega-3 and Omega-6 Fatty Acids; Figure 1.1. Classical omega-3 and omega-6 fatty acid synthesis pathways and the role of omega-3 fatty acid in regulating health/disease markers; Overview of the Electrophysiology of the Heart; Potential Impact of Omega-3 Fatty Acids on Arrhythmogenesis; Chapter 2. Methods: Overview; Analytic Framework of This Evidence Report; Figure 2.1. Analytic Framework for Animal Studies; Figure 2.2. Analytic Framework for Intact Animal Isolated Organ and Cell Studies; Figure 2.3. Analytic Framework for Cell Culture Studies; Literature Search Strategy; Study Selection; Inclusion Criteria; Data Extraction; Format for Reporting Evidence; Methods of Analysis; Diet Classification; Data Presentation; Chapter 3. Results: Literature Search Overview; Whole Animal Studies; Whole-Animal Isolated Organ and Cell Studies; Isolated Organ and Cell Culture Studies; Chapter 4. Discussion: Whole Animal Studies; Table 4.1. Comparisons of fish-oil to Omega-6 supplementation; Table 4.2. Minimum group size to detect a 50% reduction in ventricular fibrillation; Whole-Animal/Isolated Organ and Cell Studies; Isolated Organ and Cell Culture Studies; Table 4.3. Areas for future research: Whole animal and isolated organ and cell culture studies; Study Design and Analysis Issues: Isolated Organ and Cell Culture Studies; Table 4.4. Areas for future research: Isolated organ and cell culture studies; Conclusion; References and Included Studies with Bibliography; Bibliography; Listing of Excluded Studies ________________________________________________________________________________________________ 03acro.doc Microsoft Word® Document MS Word® 2002 445KB 6 pages Contents: List of Acronyms, Abbreviations, and Parameters ________________________________________________________________________________________________ 04st120.doc Microsoft Word® Document MS Word® 2002 949KB 20 pages Contents: Summary Tables 1-20: Table 3.1. Summary of Study Design and Outcomes Evaluated in Whole-Animal Studies; Table 3.2. Summary of Study Design and Outcomes Evaluated in Whole-Animal/Isolated Organ and Cell Studies; Table 3.3. Summary of Study Design and Outcomes Evaluated in Isolated Organ and Cell Studies; Table 3.4. Total Deaths in Ischemia-Reperfusion- Induced Arrhythmia: Comparison of Rats Fed Omega-3 Fatty Acids With Controls Fed Omega-6 PUFA Oils; Table 3.5. Sensitivity Analysis on Total Deaths in Ischemia-Reperfusion-Induced Arrhythmia: Comparison of Rats Fed Omega-3 Fatty Acids With Controls Fed Omega-6 PUFA Oils; Table 3.6. Total VF Deaths: Comparison of Monkeys Fed Tuna Fish oil With Controls Fed Sunflower-Seed Oil (Omega-6 PUFA); Table 3.7. Ventricular Tachycardia in Ischemia-Induced Arrhythmias: Comparison of Rats Fed Omega-3 Fatty Acids With Controls Fed Omega-6 PUFA Oils; Table 3.8. Ventricular Tachycardia in Reperfusion-Induced Arrhythmias: Comparison of Rats Fed Omega-3 Fatty Acids With Controls Fed Omega-6 PUFA Oils; Table 3.9. Ventricular Fibrillation in Ischemia-Induced Arrhythmias: Comparison of Rats Fed Omega-3 Fatty Acids With Controls Fed Omega-6 PUFA Oils; Table 3.10. Ventricular Fibrillation in Reperfusion-Induced Arrhythmias: Comparison of Rats Fed Omega-3 Fatty Acids With Controls Fed Omega-6 PUFA Oils; Table 3.11. Ventricular Fibrillation in Induced Arrhythmia: Comparison of Monkeys Fed Tuna Fish oil With Controls Fed Sunflower-Seed Oil (Omega-6 PUFA); Table 3.12. Ventricular Premature Beats/Complex, Infarct Size, Arrhythmia Score and Length of Time in Normal Sinus Rhythm: Comparison of Rats Fed Omega-3 Fatty Acids With Controls Fed Omega-6 PUFA Oils; Table 3.13. Arrhythmic Effects in Studies Comparing Omega-3 Long-Chain PUFAs With ?-Linolenic Acid; Table 3.14. Total Deaths in Ischemia-Reperfusion- Induced Arrhythmias: Comparison of Animals Fed Fish Oil (EPA+DHA) With Controls Fed Saturated Fats; Table 3.15. Ventricular Tachycardia in Ischemia-Reperfusion-Induced Arrhythmias: Comparison of Animals Fed Fish Oil With Controls Fed Saturated Fats; Table 3.16. Ventricular Fibrillation in Ischemia-Reperfusion-Induced Arrhythmias: Comparison of Animals Fed Fish Oil With Controls Fed Saturated Fats; Table 3.17. Ventricular Premature Beats in Ischemia-Reperfusion-Induced Arrhythmias: Comparison of Animals Fed Fish Oil With Controls Fed Saturated Fats; Table 3.18. Total Deaths in Induced Arrhythmias: Comparison of Dogs Fed EPA and/or DHA With No Treatment Controls; Table 3.19. Ventricular Premature Beats/Complex, Infarct Size, Arrhythmia Score and Areas Risk of Arrhythmias: Comparison of Dogs Fed EPA and/or DHA With No Treatment Controls; Table 3.20. Effects of Intravenously Infused Omega-3 Fatty Acids on Ischemia-Induced or Spontaneous Arrhythmias in Mongrel Dogs ________________________________________________________________________________________________ 05st2131.doc Microsoft Word® Document MS Word® 2002 1.2MB 31 pages Contents: Summary Tables 21-30: Table 3.21 Effects of Omega-3 Fatty Acids on Contractile Parameters In Whole Animal Isolated Organ and Cell Studies (WA-IOC) Sorted by Diet and Conditions; Table 3.22. Effects of Omega-3 Fatty Acids on the Basoelectromechanical Parameters in WA-IOC; Table 3.23. Effects of Omega-3 Fatty Acids on Ion Pumps and Ion Movement in Whole Animal Isolated Organ and Cell Studies Sorted by Species and Diet; Table 3.24. Effects of Omega-3 Fatty Acids on Ion Currents in WA-IOC; Table 3.25. Effects of Omega-3 Fatty Acids on Ion Channels in WA-IOC; Table 3.26. Effects of Omega-3 Fatty Acids on Arrhythmogenic and Contractile Parameters in WA-IOC; Table 3.27. Effects of Omega-3 Fatty Acids on Basoelectromechanical Parameters in WA-IOC; Table 3.28. Effects of Omega-3 Fatty Acids on Ion Pumps and Ion Movement; Table 3.29 Effects of Omega-3 Fatty Acids on Ion Currents; Table 3.30. Effects of Omega-3 Fatty Acids on Ion Channels; Table 3.31. Comparison of IC50 or EC50 Values ________________________________________________________________________________________________ 06appa.doc Microsoft Word® Document MS Word® 2002 36KB 7 pages Contents: Search Strategies: A.1 Primary Search Strategy; A.2 Diabetes Search Strategy; A.3 Nut Search Strategy; A.4 Risk Factor Update Search Strategy ________________________________________________________________________________________________ 07appb.doc Microsoft Word® Document MS Word® 2002 638KB 7 pages Contents: Whole Animal Result Form ________________________________________________________________________________________________ 08appct1.doc Microsoft Word® Document MS Word® 2002 265KB 19 pages Contents: Appendix C: Evidence Table 1. Whole Animal Studies ________________________________________________________________________________________________ 09appct2.doc Microsoft Word® Document MS Word® 2002 316KB 14 pages Contents: Appendix C: Evidence Table 2. Whole-Animal Isolated Organ and Cell Studies ________________________________________________________________________________________________ 10appct3.doc Microsoft Word® Document MS Word® 2002 716KB 27 pages Contents: Appendix C: Evidence Table 3. Isolated Organ And Cell Culture Studies ________________________________________________________________________________________________ 11appd.doc Microsoft Word® Document MS Word® 2002 31KB 1 page Contents: Appendix D: Peer Reviewers ________________________________________________________________________________________________
AHRQ Publication No. 04-E011-2
Current as of March 2004
Internet Citation:
Effects of Omega-3 Fatty Acids on Arrhythmogenic Mechanisms in Animal and Isolated Organ/Cell Culture Studies. File Inventory, Evidence Report/Technology Assessment Number 92. AHRQ Publication No. 04-E011-2, March 2004. Agency for Healthcare Research and Quality, Rockville, MD. http://www.ahrq.gov/clinic/o3arrinv.htm
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