Evidence Report/Technology Assessment Number 92

Effects of Omega-3 Fatty Acids on Arrhythmogenic Mechanisms in Animal and Isolated Organ/Cell Culture Studies

File Inventory


Prepared by:

Tufts-New England Medical Center Evidence-based Practice Center
Boston, Massachusetts

Investigators
   Harmon Jordan, Sc.D., Project Leader
   Nirupa Matthan, Ph.D., Co-Project Leader
   Mei Chung, M.P.H., Research Associate
   Ethan Balk, M.D., M.P.H., Methodologist
   Priscilla Chew, M.D., Research Associate
   Bruce Kupelnick, B.A., Research Associate
   Deirdre DeVine, M.Litt., Project Manager
   Amy Lawrence, B.A., Research Assistant
   Alice Lichtenstein, D.Sc., Primary Technical Expert
   Joseph Lau, M.D., Principal Investigator


File Name	Description			Software	Version	File	Size
________________________________________________________________________________________________
01front.doc	Microsoft Word® Document	MS Word®	2002	87KB	14 pages
Contents: Title Page, Preface, Acknowledgments, Technical Expert Panel, Structured Abstract, 
Table of Contents
________________________________________________________________________________________________
02body.doc	Microsoft Word® Document	MS Word®	2002	1.6MB	74 pages
Contents: Chapter 1. Introduction: Metabolism and Biological Effects of Essential Fatty Acids; 
  Metabolic Pathways of Omega-3 and Omega-6 Fatty Acids; Figure 1.1. Classical omega-3 and 
  omega-6 fatty acid synthesis pathways and the role of omega-3 fatty acid in regulating 
  health/disease markers; Overview of the Electrophysiology of the Heart; Potential Impact 
  of Omega-3 Fatty Acids on Arrhythmogenesis; 
Chapter 2. Methods: Overview; Analytic Framework of This Evidence Report; Figure 2.1. 
  Analytic Framework for Animal Studies; Figure 2.2. Analytic Framework for Intact Animal 
  Isolated Organ and Cell Studies; Figure 2.3. Analytic Framework for Cell Culture Studies; 
  Literature Search Strategy; Study Selection; Inclusion Criteria; Data Extraction; 
  Format for Reporting Evidence; Methods of Analysis; Diet Classification; Data Presentation; 
Chapter 3. Results: Literature Search Overview; Whole Animal Studies; Whole-Animal Isolated 
  Organ and Cell Studies; Isolated Organ and Cell Culture Studies; 
Chapter 4. Discussion: Whole Animal Studies; Table 4.1. Comparisons of fish-oil to Omega-6 
  supplementation; Table 4.2. Minimum group size to detect a 50% reduction in ventricular 
  fibrillation; Whole-Animal/Isolated Organ and Cell Studies; Isolated Organ and Cell Culture 
  Studies; Table 4.3. Areas for future research: Whole animal and isolated organ and cell 
  culture studies; Study Design and Analysis Issues: Isolated Organ and Cell Culture Studies; 
  Table 4.4. Areas for future research: Isolated organ and cell culture studies; Conclusion; 
References and Included Studies with Bibliography; Bibliography; Listing of Excluded Studies
________________________________________________________________________________________________
03acro.doc	Microsoft Word® Document	MS Word®	2002	445KB	6 pages
Contents: List of Acronyms, Abbreviations, and Parameters
________________________________________________________________________________________________
04st120.doc	Microsoft Word® Document	MS Word®	2002	949KB	20 pages
Contents: Summary Tables 1-20: Table 3.1. Summary of Study Design and Outcomes Evaluated in 
Whole-Animal Studies; Table 3.2. Summary of Study Design and Outcomes Evaluated in 
Whole-Animal/Isolated Organ and Cell Studies; Table 3.3. Summary of Study Design and Outcomes 
Evaluated in Isolated Organ and Cell Studies; Table 3.4. Total Deaths in Ischemia-Reperfusion-
Induced Arrhythmia: Comparison of Rats Fed Omega-3 Fatty Acids With Controls Fed Omega-6 PUFA 
Oils; Table 3.5. Sensitivity Analysis on Total Deaths in Ischemia-Reperfusion-Induced Arrhythmia: 
Comparison of Rats Fed Omega-3 Fatty Acids With Controls Fed Omega-6 PUFA Oils; Table 3.6. 
Total VF Deaths: Comparison of Monkeys Fed Tuna Fish oil With Controls Fed Sunflower-Seed Oil 
(Omega-6 PUFA); Table 3.7. Ventricular Tachycardia in Ischemia-Induced Arrhythmias: Comparison
of Rats Fed Omega-3 Fatty Acids With Controls Fed Omega-6 PUFA Oils; Table 3.8. Ventricular 
Tachycardia in Reperfusion-Induced Arrhythmias: Comparison of Rats Fed Omega-3 Fatty Acids 
With Controls Fed Omega-6 PUFA Oils; Table 3.9. Ventricular Fibrillation in Ischemia-Induced 
Arrhythmias: Comparison of Rats Fed Omega-3 Fatty Acids With Controls Fed Omega-6 PUFA Oils; 
Table 3.10. Ventricular Fibrillation in Reperfusion-Induced Arrhythmias: Comparison of Rats 
Fed Omega-3 Fatty Acids With Controls Fed Omega-6 PUFA Oils; Table 3.11. Ventricular Fibrillation 
in Induced Arrhythmia: Comparison of Monkeys Fed Tuna Fish oil With Controls Fed Sunflower-Seed 
Oil (Omega-6 PUFA); Table 3.12. Ventricular Premature Beats/Complex, Infarct Size, Arrhythmia 
Score and Length of Time in Normal Sinus Rhythm: Comparison of Rats Fed Omega-3 Fatty Acids 
With Controls Fed Omega-6 PUFA Oils; Table 3.13. Arrhythmic Effects in Studies Comparing 
Omega-3 Long-Chain PUFAs With ?-Linolenic Acid; Table 3.14. Total Deaths in Ischemia-Reperfusion-
Induced Arrhythmias: Comparison of Animals Fed Fish Oil (EPA+DHA) With Controls Fed Saturated Fats; 
Table 3.15. Ventricular Tachycardia in Ischemia-Reperfusion-Induced Arrhythmias: Comparison of 
Animals Fed Fish Oil With Controls Fed Saturated Fats; Table 3.16. Ventricular Fibrillation in 
Ischemia-Reperfusion-Induced Arrhythmias: Comparison of Animals Fed Fish Oil With Controls Fed 
Saturated Fats; Table 3.17. Ventricular Premature Beats in Ischemia-Reperfusion-Induced 
Arrhythmias: Comparison of Animals Fed Fish Oil With Controls Fed Saturated Fats; Table 3.18. 
Total Deaths in Induced Arrhythmias: Comparison of Dogs Fed EPA and/or DHA With No Treatment 
Controls; Table 3.19. Ventricular Premature Beats/Complex, Infarct Size, Arrhythmia Score and 
Areas Risk of Arrhythmias: Comparison of Dogs Fed EPA and/or DHA With No Treatment Controls; 
Table 3.20. Effects of Intravenously Infused Omega-3 Fatty Acids on Ischemia-Induced or 
Spontaneous Arrhythmias in Mongrel Dogs
________________________________________________________________________________________________
05st2131.doc	Microsoft Word® Document	MS Word®	2002	1.2MB	31 pages
Contents: Summary Tables 21-30: Table 3.21   Effects of Omega-3 Fatty Acids on Contractile 
Parameters In Whole Animal Isolated Organ and Cell Studies (WA-IOC) Sorted by Diet and 
Conditions; Table 3.22. Effects of Omega-3 Fatty Acids on the Basoelectromechanical Parameters 
in WA-IOC; Table 3.23. Effects of Omega-3 Fatty Acids on Ion Pumps and Ion Movement in Whole 
Animal Isolated Organ and Cell Studies Sorted by Species and Diet; Table 3.24. Effects of 
Omega-3 Fatty Acids on Ion Currents in WA-IOC; Table 3.25. Effects of Omega-3 Fatty Acids 
on Ion Channels in WA-IOC; Table 3.26. Effects of Omega-3 Fatty Acids on Arrhythmogenic and 
Contractile Parameters in WA-IOC; Table 3.27. Effects of Omega-3 Fatty Acids on 
Basoelectromechanical Parameters in WA-IOC; Table 3.28. Effects of Omega-3 Fatty Acids on 
Ion Pumps and Ion Movement; Table 3.29 Effects of Omega-3 Fatty Acids on Ion Currents; 
Table 3.30. Effects of Omega-3 Fatty Acids on Ion Channels; Table 3.31. Comparison of IC50 
or EC50 Values
________________________________________________________________________________________________
06appa.doc	Microsoft Word® Document	MS Word®	2002	36KB	7 pages
Contents: Search Strategies: A.1 Primary Search Strategy; A.2 Diabetes Search Strategy; 
A.3 Nut Search Strategy; A.4 Risk Factor Update Search Strategy
________________________________________________________________________________________________
07appb.doc	Microsoft Word® Document	MS Word®	2002	638KB	7 pages
Contents: Whole Animal Result Form
________________________________________________________________________________________________
08appct1.doc	Microsoft Word® Document	MS Word®	2002	265KB	19 pages
Contents: Appendix C: Evidence Table 1. Whole Animal Studies
________________________________________________________________________________________________
09appct2.doc	Microsoft Word® Document	MS Word®	2002	316KB	14 pages
Contents: Appendix C: Evidence Table 2. Whole-Animal Isolated Organ and Cell Studies
________________________________________________________________________________________________
10appct3.doc	Microsoft Word® Document	MS Word®	2002	716KB	27 pages
Contents: Appendix C: Evidence Table 3. Isolated Organ And Cell Culture Studies
________________________________________________________________________________________________
11appd.doc	Microsoft Word® Document	MS Word®	2002	31KB	1 page
Contents: Appendix D: Peer Reviewers
________________________________________________________________________________________________

AHRQ Publication No. 04-E011-2
Current as of March 2004


Internet Citation:

Effects of Omega-3 Fatty Acids on Arrhythmogenic Mechanisms in Animal and Isolated Organ/Cell Culture Studies. File Inventory, Evidence Report/Technology Assessment Number 92. AHRQ Publication No. 04-E011-2, March 2004. Agency for Healthcare Research and Quality, Rockville, MD. http://www.ahrq.gov/clinic/o3arrinv.htm


Return to Evidence-based Practice
Clinical Information
AHRQ Home Page
Department of Health and Human Services