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Assay for Enterohemorrhagic Escherichia Coli 0157:H7 by the Polymerase Chain Reaction
Patent Number: 5652102
Docket Number: 7994
Serial Number: 8357791
Date Patented: 07/29/1997
Agency:
Agricultural Research Service
Date Issued:
July 29, 1997
Abstract:
The invention involves the use of primers as a reliable diagnostic tool to detect the
pathogen Escherichia coli 0157:H7 by polymerase chain reaction methods. The
primers are derived from DNA sequences contained within a specific plasmid,
which is present in most E. coli. The invention may also be used in combination
with primers derived from other sequences, such as shiga-like toxins I and II and
the eaeA gene to specifically identify E. coli.
Escherichia coli 0157:H7, also known as enterohemorrhagic E. coli (EHEC), has
been associated with recent outbreaks of food borne disease. Sporadic cases of
hemorrhagic colitis and hemolytic uremic syndrome have occurred worldwide.
These outbreaks have been attributed to the presence of E. coli in ground beef. The
most visible outbreak in the United States occurred in the Western states involving
more than 500 cases and resulting in the deaths of several young children.
Inventors:
| Pina M. Fratamico | Solomon K. Sackitey |
| Eastern Regional Research Center | (Same as first inventor) |
| 600 East Mermaid Lane | (215) 233-6524/(215) 233-6581 (fax) |
| Wyndmoor, PA 19118 |
| (215)233-6525/(215)233-6581 (fax) |
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| Martin Wiedmann |
| Cornell University |
| Department of Food Science |
| Ithaca, NY 14853 |
| (215) 233-6620 |
Industry Keywords:
meat, food safety, biotechnology, medical, diagnostic, nucleic acids, ground
beef, genes, microorganisms, pathogen, bacteria, E. coli
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