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MONOCLONAL ANTIBODIES TO POTATO AND TOMATO GLYCOALKALOIDS AND ASSAYS FOR THE SAME
Patent Number: 5614408
Docket Number: 19494
Serial Number: 8544748
Date Patented: 03/25/1997
Agency:
Agricultural Research Service
Issued:
March 25, 1997
Abstract:
The invention is a way to detect and quantify glycoalkaloids, natural toxins that are found
in potatoes, tomatoes, and eggplants. A hybridoma cell line produces and secretes
monoclonal antibodies that selectively bind to the glycoalkaloids, as well as their
corresponding aglycones. Detection of glycoalkaloids in potatoes, tomatoes, and/or
eggplants in biological samples may occur by contacting the sample with the antibodies to
form a detectable glycoalkaloid/ antibody immunocomplex when the glycoalkaloids are
present. The monoclonal antibody may also be incorporated into kits for detecting and
quantifying glycoalkaloids in plants, foods, and body tissues and fluids.
Inventors:
| Larry H. Stanker | Carol K. Holtzapple |
| USDA, ARS, SPA | USDA, ARS, SPA |
| College Station, TX 77845 | College Station, TX 77845 |
| (409) 260-9306 | (409) 260-9263 |
| (409) 260-9332 (Fax) | (409) 260-9332 (Fax) |
Industry Keywords:
cell lines, hybridomas, monoclonal antibodies, food safety, toxins, diagnostic, potatoes,
tomatoes, plant breeders, vegetables, biotechnology
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