Agency:

Agricultural Research Service

February 20, 1996

The invention uses hybridoma cell lines and monoclonal antibodies to detect the antibiotic, ceftiofur, in meat and milk. Ceftiofur is a broad spectrum cephalosporin exhibits excellent antimicrobial activity and is used in the treatment of respitory diseases in animals. The monoclonal antibodies may be incorporated into kits for detection and quantification of ceftiofur. The invention replaces expensive and sophisticated equipment currently used to test trace levels of ceftiofur in milk or meat. The invention also requires less labor that current tests, allowing producers to better manage the use of ceftiofur.

Larry H. Stanker	Beate G. Rose
USDA, ARS, SPA	1521 E. Franklin Street, B203
2881 F&B; Road	Chapel Hill NC 27514
College Station, TX 77845	(919) 966-4294
(409) 260-9306	(919) 966-0704
(409) 260-9332 -- fax

Industry Keywords:

biotechnology, drug residue, milk, meat, diagnostic, microbial, antibiotic, antibody, amino assay, ELISA, food, veterinary, beef, dairy, cattle, horse, pig, swine, chicken, antibody, amino assay, ELISA, food, veterinary, beef, dairy, cattle, horse, pig, swine, chicken, respiratory disease respiratory disease