Technology Description:

The invention relates to a method for obtaining targeted DNA in less time with greater efficiency byeliminating genomic DNA library preparation and screening. This method reduces target DNA productionfrom months to days. The targeted DNA sequence prepared by this method can be used as a template foramplification procedures such as PCR, or can be employed as a molecular marker. Examples of target DNA forwhich this method will apply include simple sequence repeats (SSRs), footprints remaining from retroviralexcision, transposable elements, or any DNA element of a length that is too short to be identified and/orisolated by conventional PCR. The method uses a polymerase having strand displacement capability, oneprimer, and a circular DNA template.

Companies engaged in the manufacture of biological markers and diagnostic kits would be interested in the technology.  Biotechnology companies would also benefit from the technology.

Reference:

Please refer to USPN 6,054,300 (Docket #0115.97), which issued April 25, 2000.  Foreign rights are available.

Inventor:

William L. McKendree
Horticultural Research Laboratory
2199 S. Rock Road
Fort Pierce, Florida 34945
(561) 462-5943