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Pro Asp 91 Leu Pro Tyr Asp Tyr Ala Asp Leu Glu Pro Val Ile Ser His Glu 136 Ile Met Gln Leu His His Gln Lys His His Ala Thr Tyr Val Asn 181 Asn Leu Asn Gln Ile Glu Glu Lys Leu His Glu Ala Val Ser Lys 226 Gly Asn Val Lys Glu Ala Ile Ala Leu Gln Pro Ala Leu Lys Phe 271 Asn Gly Gly Gly His Ile Asn His Ser Ile Phe Trp Thr Asn Leu 316 Ala Lys Asp Gly Gly Glu Pro Ser Ala Glu Leu Leu Thr Ala Ile 361 Lys Ser Asp Phe Gly Ser Leu Asp Asn Leu Gln Lys Gln Leu Ser 406 Ala Ser Thr Val Ala Val Gln Gly Ser Gly Trp Gly Trp Leu Gly 451 Tyr Cys Pro Lys Gly Lys Ile Leu Lys Val Ala Thr Cys Ala Asn 496 Gln Asp Pro Leu Glu Ala Thr Thr Gly Leu Val Pro Leu Phe Gly 541 Ile Asp Val Trp Glu His Ala Tyr Tyr Leu Gln Tyr Lys Asn Val 586 Arg Pro Asp Tyr Val Asn Ala Ile Trp Lys Ile Ala Asn Trp Lys 631 Asn Val Ser Glu Arg Phe Ala Lys Ala Gln Gln


Blast gives me an error: Server did not say "ok"

When you run Blast on the helix systems, the search actually runs to the computers at NCBI. As of Jan 1998, the NCBI 'experimental' servers (Cruncher and Muncher), which used to process blast jobs, have been replaced. The new system now sends blast jobs to the NCBI web server. If the server is overloaded or the network is slow, you may see the following error message:

helix% blast x89712.genpept -default

 Sending query...
 Awaiting results...

 ** Server did not say "OK".
Your options are to try again later, use the Blast email server (add '-mail' to the command line), or use Blast via the web.

The old 'blast -batch' command doesn't work anymore; to run blast in batch mode see How do I run blast searches in batch mode


Why have my alignments changed in Version 9?

The following information is taken from the GCG Newsletter, July 1997.

Protein sequence alignment programs in the Wisconsin Package use scoring matrices to make comparisons between pairs of amino acids. In Version 9