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UI-E-CQ0 (dbEST Library ID.10279)
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Organism:
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Homo sapiens
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Developmental stage:
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Adult
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Tissue:
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other
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Vector:
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pT7T3-Pac (Pharmacia) with a modified polylinker (Plasmid)
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Lab host:
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DH10B (Life Technologies) (T1 phage resistant)
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Cloning sites:
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EcoR I, Not I
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Library Description
UI-E-CQ0 is a cDNA library containing the following tissue(s):
optic nerve. The library was constructed according to Bonaldo,
Lennon and Soares, Genome Research, 6:791-806, 1996. First
strand cDNA synthesis was primed with an oligo-dT primer
containing a Not I site. Double stranded cDNA was ligated to an
EcoR I adaptor, digested with Not I, and cloned directionally
into pT7T3-Pac vector. The oligonucleotide used to prime the
synthesis of first-strand cDNA contains a library tag sequence
that is located between the Not I site and the (dT)18 tail. The
sequence tag for this library is CCATTAAGTG. This library was
created for the program, Gene Discovery in the Visual System,
supported by National Eye Institute (NEI).
Sequence Submitters
Soares, MB
University of Iowa
375 Newton Road , 4156 MEBRF, Iowa City, IA 52242, USA
References
 Bonaldo MF, et al. Normalization and subtraction: two approaches to facilitate gene discovery. Genome Res 6, 791-806 (1996).
Distribution of member sequences in UniGene
1360 of the 1571 sequences from this library have been classified into 744 UniGene entries
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Transcripts per million
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EST count
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UniGene Entry
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13970
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19
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Hs.446429
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Prostaglandin D2 synthase 21kDa (brain)
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12500
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17
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Hs.386793
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Glutathione peroxidase 3 (plasma)
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8820
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12
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Hs.406397
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Glial fibrillary acidic protein
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7350
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10
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Hs.436657
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Clusterin (complement lysis inhibitor, SP-40,40, sulfated glycoprotein 2, testosterone-repressed prostate message 2, apolipoprotein J)
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6610
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9
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Hs.247565
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Rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant)
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6610
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9
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Hs.139876
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Ribosomal protein S9
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5880
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8
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Hs.8102
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Ribosomal protein S20
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5880
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8
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Hs.132319
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Ferritin, heavy polypeptide 1
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5140
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7
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Hs.198273
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NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa
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4410
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6
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Hs.76067
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Heat shock 27kDa protein 1
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4410
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6
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Hs.51147
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Guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 1
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Relative frequency based on tpm
Download UniGene Sequences
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