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S7SNU719 (dbEST Library ID.10305)
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Organism:
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Homo sapiens
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Sex:
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M
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Tissue:
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Stomach
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Cell Line:
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SNU-719
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Cell type:
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Epithelial
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Vector:
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pCNS (Plasmid)
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Lab host:
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Top10F'
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Cloning sites:
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EcoRI, NotI
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Library Description
The poly (A)+ RNA was dephosphorylated with bacterial alkaline
phosphatase (BAP) and then decapped with tabacco acid
pyrophosphatase (TAP). The decapped intact mRNA was ligated
with DNA-RNA linker including EcoR I site by treatment of T4
RNA ligase and the first strand cDNA was synthesized from oligo
dT-selected mRNA by priming with dT-tailed vector. The
dT-tailed vector was adjusted to have about 60nt. The cDNA
vector was circularized with E. coli DNA ligase after digestion
of EcoRI which site is also included in vector. An RNA strand
converted to a DNA strand by Okayama-Berg method. The obtained
cDNA vectors were used for transformation of competent cells E.
coli Top10F' by electroporation method. The cDNA libraries
constructed by this method are full-length enriched cDNA
library.
Sequence Submitters
Kim YS
Korea Research Institute of Bioscience & Biotechnology
52 Eoeun-dong Yuseong-gu, Daejeon 305-333, South Korea
Kim NS
Korea Research Institute of Bioscience and Biotechnology
52 Eoeun-dong, Yuseong-gu, Daejeon 305-333, Republic of Korea
Distribution of member sequences in UniGene
2732 of the 2925 sequences from this library have been classified into 860 UniGene entries
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Transcripts per million
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EST count
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UniGene Entry
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19390
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53
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Hs.439552
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Eukaryotic translation elongation factor 1 alpha 1
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18660
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51
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Hs.356123
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Keratin 8
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18300
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50
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Hs.458332
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Pyrroline-5-carboxylate reductase 1
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17200
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47
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Hs.302903
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Ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
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14640
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40
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Hs.405474
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PTK2B protein tyrosine kinase 2 beta
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14640
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40
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Hs.148438
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Leucine rich repeat and fibronectin type III domain containing 4
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10980
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30
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Hs.432945
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Ankyrin repeat domain 9
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10240
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28
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Hs.406283
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Homo sapiens, clone IMAGE:6069899, mRNA
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9150
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25
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Hs.406458
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Glucose phosphate isomerase
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8050
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22
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Hs.103381
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Jumonji, AT rich interactive domain 1C (RBP2-like)
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6950
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19
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Hs.534568
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Kringle containing transmembrane protein 2
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Relative frequency based on tpm
Download UniGene Sequences
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