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UI-CF-DU1 (dbEST Library ID.10398)
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Organism:
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Homo sapiens
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Developmental stage:
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Adult
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Tissue:
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Lung
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Vector:
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pT7T3-Pac (Pharmacia) with a modified polylinker (Plasmid)
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Lab host:
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DH10B (Life Technologies) (T1 phage resistant)
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Cloning sites:
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EcoR I, Not I
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Library Description
UI-CF-DU1 is a normalized cDNA library containing the following
tissue(s): Primary Lung Epithelial Cells The library was
constructed according to Bonaldo, Lennon and Soares, Genome
Research, 6:791-806, 1996. First strand cDNA synthesis was
primed with an oligo-dT primer containing a Not I site. Double
stranded cDNA was ligated to an EcoR I adaptor, digested with
Not I, and cloned directionally into pT7T3-Pac vector. The
oligonucleotide used to prime the synthesis of first-strand
cDNA contains a library tag sequence that is located between
the Not I site and the (dT)18 tail. The sequence tag for this
library is GGCTGTAGGC.
Sequence Submitters
McCray, PB
University of Iowa
2024 University of Iowa Med Labs, Iowa City, IA 52242, USA
References
 Bonaldo MF, et al. Normalization and subtraction: two approaches to facilitate gene discovery. Genome Res 6, 791-806 (1996).
Distribution of member sequences in UniGene
10821 of the 12742 sequences from this library have been classified into 5249 UniGene entries
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Transcripts per million
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EST count
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UniGene Entry
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5082
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55
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Hs.205126
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Polymeric immunoglobulin receptor
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2587
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28
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Hs.446579
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Heat shock 90kDa protein 1, alpha
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2217
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24
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Hs.433673
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Calreticulin
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2125
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23
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Hs.406300
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Ribosomal protein L23
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2125
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23
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Hs.187199
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Metastasis associated lung adenocarcinoma transcript 1 (non-coding RNA)
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2033
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22
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Hs.274479
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ATPase, Na+/K+ transporting, beta 1 polypeptide
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2033
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22
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Hs.202166
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Heterogeneous nuclear ribonucleoprotein H1 (H)
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1663
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18
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Hs.56845
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GDP dissociation inhibitor 2
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1663
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18
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Hs.512676
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Ribosomal protein S25
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1663
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18
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Hs.426930
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Actin, beta
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1478
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16
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Hs.436687
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SET translocation (myeloid leukemia-associated)
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Relative frequency based on tpm
Download UniGene Sequences
UNIX
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MAC
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