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NCI_CGAP_Pl3 (dbEST Library ID.10405)
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Organism:
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Homo sapiens
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Developmental stage:
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Embryo
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Tissue:
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Placenta
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Vector:
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pT7T3-Pac (Pharmacia) with a modified polylinker (Plasmid)
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Lab host:
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DH10B (Life Technologies)
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Cloning sites:
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EcoR I, Not I
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Library Description
NCI_CGAP_Pl3 is a subtracted cDNA library constructed according
to Bonaldo, Lennon and Soares, Genome Research, 6:791-806,
1996. First strand cDNA synthesis was primed with an oligo-dT
primer containing a Not I site. Double stranded cDNA was
ligated to an EcoR I adaptor, digested with Not I, and cloned
directionally into pT7T3-Pac vector. The oligonucleotide used
to prime the synthesis of first-strand cDNA contains a library
tag sequence that is located between the Not I site and the
(dT)18 tail. The sequence tags for this library are GA, AGGAA.
For additional information, contact: Bento Soares,
bento-soares@uiowa.edu
Sequence Submitters
Robert Strausberg, Ph.D.
Distribution of member sequences in UniGene
3973 of the 4425 sequences from this library have been classified into 2153 UniGene entries
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Transcripts per million
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EST count
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UniGene Entry
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5280
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21
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Hs.283022
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Triggering receptor expressed on myeloid cells 1
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3770
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15
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Hs.235750
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Translocase of inner mitochondrial membrane 10 homolog (yeast)
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3520
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14
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Hs.306079
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Sec61 alpha 1 subunit (S. cerevisiae)
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3520
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14
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Hs.129477
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MRNA; cDNA DKFZp686A2436 (from clone DKFZp686A2436)
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3270
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13
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Hs.155728
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ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d
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3270
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13
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Hs.108408
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Likely ortholog of C. elegans anterior pharynx defective 1A
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3020
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12
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Hs.76285
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DKFZP564B167 protein
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3020
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12
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Hs.2210
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Thyroid hormone receptor interactor 3
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2760
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11
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Hs.79058
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Suppressor of Ty 4 homolog 1 (S. cerevisiae)
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2760
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11
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Hs.78781
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Vascular endothelial growth factor B
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2760
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11
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Hs.458425
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G protein-coupled receptor 109B
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Relative frequency based on tpm
Download UniGene Sequences
UNIX
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