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NIH_MGC_186 (dbEST Library ID.13018)
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Organism:
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Homo sapiens
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Tissue:
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mixed
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Vector:
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pDNR-LIB (plasmid (chloramphenicol resistant, grown at 30C))
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Lab host:
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DH10B (T1 phage-resistant)
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Cloning sites:
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SfiI (ggccattatggcc), SfiI (ggccgcctcggcc)
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Library Description
Library is oligo-dT primed and directionally cloned. cDNA was
prepared from a pooled samples of tissues from Skin, meninges,
duramatter, pia matter and choroid plexus. 5' and 3' adaptors
were used in cloning as follows: 5' adaptor sequence:
5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence:
5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or
G and N = A, C, G, or T). Average insert size 1.47 kb (range
0.50-4.0 kb). 15/15 colonies contained inserts by PCR. This
library was enriched for full-length clones and was constructed
by Clontech Laboratories (Palo Alto, CA). Note: this is a
NIH_MGC Library
Sequence Submitters
Robert Strausberg, Ph.D.
Daniela S. Gerhard, Ph.D.
National Cancer Institute / NIH
Bldg. 31 Rm10A07 Bethesda, MD 20892
Distribution of member sequences in UniGene
5668 of the 6795 sequences from this library have been classified into 2420 UniGene entries
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Transcripts per million
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EST count
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UniGene Entry
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264110
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1497
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Hs.427202
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Transthyretin (prealbumin, amyloidosis type I)
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11110
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63
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Hs.391561
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Fatty acid binding protein 4, adipocyte
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9700
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55
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Hs.439552
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Eukaryotic translation elongation factor 1 alpha 1
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7760
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44
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Hs.156316
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Decorin
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5640
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32
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Hs.462864
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Annexin A2
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5290
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30
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Hs.187199
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Metastasis associated lung adenocarcinoma transcript 1 (non-coding RNA)
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5110
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29
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Hs.48516
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Beta-2-microglobulin
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3880
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22
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Hs.433670
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Ferritin, light polypeptide
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3880
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22
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Hs.425808
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Calmodulin 2 (phosphorylase kinase, delta)
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3700
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21
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Hs.406283
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Homo sapiens, clone IMAGE:6069899, mRNA
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3700
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21
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Hs.287558
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Annexin A1
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Relative frequency based on tpm
Download UniGene Sequences
UNIX
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PC
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MAC
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