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NCI_CGAP_Lu24 (dbEST Library ID.1611)

Organism: Homo sapiens
Tissue: Lung
Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker (phagemid (ampicillin resistant))
Lab host: DH10B

Library Description
Plasmid DNA from the normalized library NCI_CGAP_Lu5 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1414920-1417991 and 1520904-1522439). Subtraction by Bento Soares and M. Fatima Bonaldo.

Sequence Submitters
Robert Strausberg, Ph.D.

Prange CK
Lawrence Livermore National Laboratory
Livermore, CA, USA

Ina Rolfs
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany

Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
Heubnerweg 6, D-14059 Berlin, Germany

Distribution of member sequences in UniGene
32287 of the 37215 sequences from this library have been classified into 9092 UniGene entries

Transcripts per million   EST count UniGene Entry  
16539 534 Hs.75307 H1 histone family, member X
13287 429 Hs.410497 Brain protein I3
5822 188 Hs.1619 Achaete-scute complex-like 1 (Drosophila)
2260 73 Hs.419463 Ribosomal protein L23a
1982 64 Hs.503733 Transcribed locus
1951 63 Hs.380953 Ribosomal protein L38
1920 62 Hs.442156 CCAAT/enhancer binding protein (C/EBP), delta
1858 60 Hs.120197 Protein phosphatase 1, regulatory (inhibitor) subunit 14B
1827 59 Hs.127842 Hepatoma-derived growth factor, related protein 3
1796 58 Hs.440962 Similar to 60S ribosomal protein L23a
1765 57 Hs.388927 YY1 transcription factor
   Relative frequency based on tpm


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