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Stratagene ovary (#937217) (dbEST Library ID.253)

Organism: Homo sapiens
Developmental stage: 49 year old
Sex: female
Tissue: Ovary
Vector: Bluescript SK (phagemid)
Lab host: SOLR cells (kanamycin resistant)
Cloning sites: EcoRI, XhoI

Library Description
Cloned unidirectionally. Primer: Oligo dT. Total ovary tissue, normal, caucasian. Average insert size: 0.8 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

Sequence Submitters
Wilson RK
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108

Robert Strausberg, Ph.D.

Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
Heubnerweg 6, D-14059 Berlin, Germany

References
Hillier LD, et al. Generation and analysis of 280,000 human expressed sequence tags. Genome Res 6, 807-828 (1996).

Distribution of member sequences in UniGene
2421 of the 4017 sequences from this library have been classified into 1100 UniGene entries

Transcripts per million   EST count UniGene Entry  
15690 38 Hs.356572 Ribosomal protein S3A
11970 29 Hs.387576 Ribosomal protein S3
10730 26 Hs.119598 Ribosomal protein L3
9910 24 Hs.420233 Ribosomal protein L9
9080 22 Hs.439552 Eukaryotic translation elongation factor 1 alpha 1
8670 21 Hs.412370 Ribosomal protein L9
8670 21 Hs.406300 Ribosomal protein L23
8670 21 Hs.311640 Ribosomal protein S27a
8260 20 Hs.8102 Ribosomal protein S20
8260 20 Hs.447524 Similar to ribosomal protein S3a; 40S ribosomal protein S3a; v-fos transformation effector protein 1
7020 17 Hs.184582 Ribosomal protein L24
   Relative frequency based on tpm


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