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Soares fetal liver spleen 1NFLS (dbEST Library ID.262)
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Organism:
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Homo sapiens
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Developmental stage:
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Embryo
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Sex:
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male
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Tissue:
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mixed
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Vector:
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pT7T3D (Pharmacia) with a modified polylinker (phagemid)
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Lab host:
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DH10B (ampicillin resistant)
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Cloning sites:
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Pac I, Eco RI
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Library Description
1st strand cDNA was primed with a Pac I - oligo(dT) primer [5'
AACTGGAAGAATTAATTAAAGATCTTTTTTTTTTTTTTTTTTT 3'],
double-stranded cDNA was ligated to Eco RI adaptors
(Pharmacia), digested with Pac I and cloned into the Pac I and
Eco RI sites of the modified pT7T3 vector. Library went through
one round of normalization. Library constructed by Bento Soares
and M.Fatima Bonaldo.
Sequence Submitters
Bento Soares
Columbia University
Department of Psychiatry,722 W 168th Street, Unit #41, New York,NY 10032
Wilson RK
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
Keen TJ
Institute of Ophthalmology
Bath St., London, EC1V 9EL
Hamilton, Robert
Mississippi College
200 South Capitol Street, Clinton, MS 39058, USA
Felix, Carolyn A.
Joseph Stokes, Jr. Research Institute, Children s Hospital of Philadelphia, University of Pennsylvania School of Medicine
Leonard and Madlyn Abramson Pediatric Research Center, Rm. 902B, 324 South 34th St., Philadelphia, PA 19104-4318, USA
Timms,K.M., Lu,F., Shen,Y., Pierson,C.A., Muzny,D.M., Gu,Y., Nelson,D.L. and Gibbs,R.A.
Baylor College of Medicine
Houston, TX 77030, USA
Jiang, Ning
Cancer Research Institute, Hunan Medical University
Xiangya Road 88, Changsha, Hunan 410078, P.R.China
Driouch K
Centre Rene Huguenin
35 rue Dailly, F-92211 St-Cloud, France
Watkins F
Oxford University
John Radcliffe Hospital, Oxford, OX3 9DU, UK
Scherer, S.W.; Rommens, J.M.; Tsui, L.C.; Nakabayashi, K.
Department of Genetics, The Hospital for Sick Children
555 University Avenue, Toronto, Ontario M5G 1X8, Canada
Ina Rolfs
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany
Bryan Williams
Lerner Research Institute
NB40, 9500 Euclid Ave., Cleveland, OH 44195, USA
Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
Heubnerweg 6, D-14059 Berlin, Germany
References
 Timms KM, et al. 130 kb of DNA sequence reveals two new genes and a regional duplication distal to the human iduronate-2-sulfate sulfatase locus. Genome Res 5, 71-78 (1995).
Hillier LD, et al. Generation and analysis of 280,000 human expressed sequence tags. Genome Res 6, 807-828 (1996).
Felix CA, et al. Panhandle polymerase chain reaction amplifies MLL genomic translocation breakpoint involving unknown partner gene. Blood 90, 4679-4686 (1997).
Distribution of member sequences in UniGene
67623 of the 91525 sequences from this library have been classified into 11368 UniGene entries
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Transcripts per million
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EST count
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UniGene Entry
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18041
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1220
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Hs.398636
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Hemoglobin, alpha 2
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16902
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1143
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Hs.302145
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Hemoglobin, gamma G
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11948
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808
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Hs.449631
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Hemoglobin, gamma A
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5915
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400
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Hs.297681
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Serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
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5870
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397
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Hs.449630
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Hemoglobin, alpha 1
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4037
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273
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Hs.251664
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Insulin-like growth factor 2 (somatomedin A)
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4022
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272
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Hs.415722
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H19, imprinted maternally expressed untranslated mRNA
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3667
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248
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Hs.155376
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Hemoglobin, beta
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3164
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214
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Hs.418167
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Albumin
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3001
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203
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Hs.523407
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H19, imprinted maternally expressed untranslated mRNA
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2942
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199
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Hs.237658
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Apolipoprotein A-II
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Relative frequency based on tpm
Download UniGene Sequences
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