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NCI_CGAP_Sub5 (dbEST Library ID.2817)
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Organism:
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Homo sapiens
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Tissue:
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other
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Vector:
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pT7T3D-Pac (Pharmacia) with a modified polylinker (Phagemid)
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Lab host:
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DH10B (Life Technologies)
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Cloning sites:
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Not I, Eco RI
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Library Description
NCI_CGAP_Sub5 is a subtracted library derived from
NCI_CGAP_Sub4. The NCI_CGAP_Sub5 library had 3 million
recombinants. A single-stranded DNA preparation of
NCI_CGAP_Sub4 was used as a tracer in a subtractive
hybridization with a driver comprising: the IMAGE pool
(NCI_CGAP_Kid3 pool 1 LLAM 3334-3337, 3682-3683, 3798-3803
(IMAGE CloneIDs 1322376-1323911,
1456008-1456775,1500552-1502855); NCI_CGAP_Kid5 pool 1 LLAM
3338-3342,3722-3725, 3776-3778 (IMAGE CloneIDs 1323912-1325831,
1471368-1472903, 1492104-1493255); NCI_CGAP_Lu5 pool 1 LLAM
3575-3582, 3851-3854 (IMAGE CloneIDs
1414920-1417991,1520904-1522439); NCI_CGAP_GC4 pool 1 LLAM
3164-3167, 3716-3720, 3733-3735 (IMAGE CloneIDs
1257096-1258631,1469064-1470983, 1475592-1476743);
NCI_CGAP_Pr22 pool 1 LLAM 2457-2459, 2758-2759, 3062-3068
(IMAGE CloneIDs 985608-986759,1101192-1101959,
1217928-1220615); NCI_CGAP_Co10 pool 1 LLAM 2644-2653,
2871-2872 (IMAGE CloneIDs 1057416-1061255,1144584-1145351).
(10% of the driver population), plus a pool of 3,840 arrayed
clones from NCI_CGAP_Sub1 (IMAGE CloneIDs 2708616-2710535) and
NCI_CGAP_Sub2 (IMAGE CloneIDs 2710536-2712455) (10% of the
driver population), plus a pool of 11,136 clones from
NCI_CGAP_Sub3 (IMAGE CloneIDs 2712456-2723591) (10% of the
driver population), plus a pool of 5,472 clones from
NCI_CGAP_Sub4 (IMAGE CloneIDs 2723592-2728969) (70% of the
driver population). Subtraction was performed as previously
described [Bonaldo, Lennon & Soares (1996): Normalization and
Subtraction: Two Approaches To Facilitate Gene Discovery.
Genome Research 6, 791-806.
Sequence Submitters
Robert Strausberg, Ph.D.
Ina Rolfs
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany
Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
Heubnerweg 6, D-14059 Berlin, Germany
Distribution of member sequences in UniGene
4071 of the 4995 sequences from this library have been classified into 3336 UniGene entries
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Transcripts per million
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EST count
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UniGene Entry
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2940
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12
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Hs.127686
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Interferon regulatory factor 4
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2210
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9
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Hs.118483
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Myosin VI
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1470
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6
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Hs.18879
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Chromosome 12 open reading frame 22
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1470
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6
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Hs.17270
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G protein-coupled receptor 124
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1220
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5
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Hs.89633
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Promyelocytic leukemia
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1220
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5
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Hs.418097
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Chromosome 9 open reading frame 5
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1220
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5
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Hs.112237
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Myosin IXA
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980
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4
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Hs.521087
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Solute carrier family 12 (potassium/chloride transporters), member 9
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980
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4
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Hs.444460
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Hypothetical protein DKFZp761A052
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980
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4
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Hs.409194
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Tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase
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980
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4
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Hs.402727
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Ankyrin repeat domain 11
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Relative frequency based on tpm
Download UniGene Sequences
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