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Soares adult brain N2b4HB55Y (dbEST Library ID.299)
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Organism:
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Homo sapiens
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Developmental stage:
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55-year old
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Sex:
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Male
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Tissue:
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Brain
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Vector:
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pT7T3D (Pharmacia) with a modified polylinker (phagemid)
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Lab host:
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DH10B (ampicillin resistant)
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Cloning sites:
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Not I, Eco RI
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Library Description
1st strand cDNA was primed with a Not I - oligo(dT) primer [5'
TGTTACCAATCTGAAGTGGGAGCGGCCGCGCTTTTTTTTTTTTTTTTTTT 3'],
double-stranded cDNA was size selected, ligated to Eco RI
adapters (Pharmacia), digested with Not I and cloned into the
Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia).
Library went through one round of normalization to a Cot = 53.
Library constructed by Bento Soares and M.Fatima Bonaldo. The
adult brain RNA was provided by Dr. Donald H. Gilden. Tissue
was acquired 17-18 hours after death which occurred in
consequence of a ruptured aortic aneurysm. RNA was prepared
from a pool of tissues representing the following areas of the
brain: frontal, parietal, temporal and occipital cortex from
the left and right hemispheres, subcortical white matter, basal
ganglia, thalamus, cerebellum, midbrain, pons and medulla.
Sequence Submitters
Wilson RK
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
Ina Rolfs
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany
Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
Heubnerweg 6, D-14059 Berlin, Germany
References
 Hillier LD, et al. Generation and analysis of 280,000 human expressed sequence tags. Genome Res 6, 807-828 (1996).
Distribution of member sequences in UniGene
3639 of the 4512 sequences from this library have been classified into 1434 UniGene entries
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Transcripts per million
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EST count
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UniGene Entry
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14010
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51
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Hs.169476
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Glyceraldehyde-3-phosphate dehydrogenase
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10440
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38
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Hs.74576
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GDP dissociation inhibitor 1
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7960
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29
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Hs.369110
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Cyclin I
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6040
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22
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Hs.32374
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Deltex 3 homolog (Drosophila)
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6040
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22
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Hs.231840
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WW domain binding protein 2
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5490
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20
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Hs.7158
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Ring finger protein 167
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5220
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19
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Hs.433892
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Adaptor-related protein complex 2, mu 1 subunit
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4670
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17
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Hs.79358
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Testis-specific kinase 1
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4670
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17
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Hs.267871
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ATPase, H+ transporting, lysosomal V0 subunit a isoform 1
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4390
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16
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Hs.406693
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Ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivity complementing)
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4390
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16
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Hs.182859
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Fas apoptotic inhibitory molecule 2
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Relative frequency based on tpm
Download UniGene Sequences
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