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NIH_MGC_51 (dbEST Library ID.3719)

Organism: Homo sapiens
Tissue: Lymph_Node
Cell Line: MGC85
Cell type: germinal center B cells
Vector: pT7T3-Pac (plasmid)
Lab host: DH10B (LTI)
Cloning sites: NotI, Eco RI

Library Description
Constructed from size fractionated cytoplasmic mRNA (4.4-7.4kb). Directionally cloned. Cells provided by Louis M. Staudt, Ph.D. Library preparation by Maria de Fatima Bonaldo, Ph.D. and M. Bento Soares, Ph.D.

Sequence Submitters
Robert Strausberg, Ph.D.

Soares, MB
University of Iowa
375 Newton Road , 4156 MEBRF, Iowa City, IA 52242, USA

Distribution of member sequences in UniGene
1539 of the 2381 sequences from this library have been classified into 646 UniGene entries

Transcripts per million   EST count UniGene Entry  
13640 21 Hs.246112 Activating signal cointegrator 1 complex subunit 3-like 1
9090 14 Hs.80905 Ras association (RalGDS/AF-6) domain family 2
9090 14 Hs.292119 Nucleoporin 210
8440 13 Hs.211201 Cytoplasmic FMR1 interacting protein 2
7790 12 Hs.7957 Adenosine deaminase, RNA-specific
7790 12 Hs.45719 Protein phosphatase 1, regulatory (inhibitor) subunit 16B
7140 11 Hs.499355 KIAA0226 gene product
7140 11 Hs.375001 Talin 1
7140 11 Hs.23158 F-box protein 41
5840 9 Hs.83190 Fatty acid synthase
5840 9 Hs.534422 CUG triplet repeat, RNA binding protein 1
   Relative frequency based on tpm


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