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NIH_MGC_21 (dbEST Library ID.4070)
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Organism:
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Homo sapiens
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Tissue:
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Placenta
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Vector:
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pOTB7 (plasmid (chloramphenicol resistant, grown at 30C))
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Lab host:
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DH10B (phage-resistant)
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Cloning sites:
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XhoI, EcoRI
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Library Description
cDNA made by oligo-dT priming. Directionally cloned into
EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G).
Size-selected >500bp for average insert size 1.8kb. Library
constructed by Ling Hong in the laboratory of Gerald M. Rubin
(University of California, Berkeley) using ZAP-cDNA synthesis
kit (Stratagene) and Superscript II RT (Life Technologies).
Sequence Submitters
Robert Strausberg, Ph.D.
Prange CK
Lawrence Livermore National Laboratory
Livermore, CA, USA
Ina Rolfs
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany
Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
Heubnerweg 6, D-14059 Berlin, Germany
Distribution of member sequences in UniGene
21408 of the 23681 sequences from this library have been classified into 4216 UniGene entries
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Transcripts per million
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EST count
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UniGene Entry
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14060
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301
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Hs.169476
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Glyceraldehyde-3-phosphate dehydrogenase
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13219
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283
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Hs.498569
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Ribosomal protein S2
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11023
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236
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Hs.234489
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Lactate dehydrogenase B
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10089
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216
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Hs.14376
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Actin, gamma 1
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9342
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200
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Hs.433455
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Enolase 1, (alpha)
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8875
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190
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Hs.406013
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Keratin 18
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8688
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186
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Hs.57301
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High mobility group AT-hook 1
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8501
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182
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Hs.356572
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Ribosomal protein S3A
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8081
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173
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Hs.119598
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Ribosomal protein L3
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7427
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159
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Hs.446608
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Tubulin, alpha, ubiquitous
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7146
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153
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Hs.309517
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Keratin 19
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Relative frequency based on tpm
Download UniGene Sequences
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