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Pancreatic Islet (dbEST Library ID.422)

Organism: Homo sapiens
Tissue: other
Vector: pBluescript SK- (phagemid (ampicillin resistant))
Lab host: SOLR cells (kanamycin resistant)
Cloning sites: EcoRI, XhoI

Library Description
Reference: Hum Mol Gen 2, 1795 (1993) Takeda et al. Cloned unidirectionally. Primer: Oligo dT. ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

Sequence Submitters
Wilson RK
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108

References
Hillier LD, et al. Generation and analysis of 280,000 human expressed sequence tags. Genome Res 6, 807-828 (1996).

Distribution of member sequences in UniGene
1741 of the 2013 sequences from this library have been classified into 785 UniGene entries

Transcripts per million   EST count UniGene Entry  
17230 30 Hs.439552 Eukaryotic translation elongation factor 1 alpha 1
17230 30 Hs.2879 Carboxypeptidase A1 (pancreatic)
13780 24 Hs.181289 Elastase 3B, pancreatic
9190 16 Hs.306278 CD44 antigen (homing function and Indian blood group system)
8040 14 Hs.624 Interleukin 8
8040 14 Hs.534362 Protease, serine, 1 (trypsin 1)
8040 14 Hs.367767 Protease, serine, 2 (trypsin 2)
7460 13 Hs.437028 Carboxypeptidase B1 (tissue)
6310 11 Hs.49407 Regenerating islet-derived 1 alpha (pancreatic stone protein, pancreatic thread protein)
6310 11 Hs.471034 Elastase 3A, pancreatic (protease E)
6310 11 Hs.388547 Serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3
   Relative frequency based on tpm


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