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NIH_MGC_9 (dbEST Library ID.4908)
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Organism:
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Homo sapiens
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Tissue:
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Ovary
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Vector:
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pOTB7 (plasmid (chloramphenicol resistant, grown at 30C))
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Lab host:
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DH10B (phage-resistant)
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Cloning sites:
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XhoI, EcoRI
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Library Description
cDNA made by oligo-dT priming. Directionally cloned into
EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G).
Size-selected >500bp for average insert size 1.8kb. Library
constructed by Ling Hong in the laboratory of Gerald M. Rubin
(University of California, Berkeley) using ZAP-cDNA synthesis
kit (Stratagene) and Superscript II RT (Life Technologies).
Sequence Submitters
Robert Strausberg, Ph.D.
Ina Rolfs
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany
Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
Heubnerweg 6, D-14059 Berlin, Germany
Distribution of member sequences in UniGene
11821 of the 12849 sequences from this library have been classified into 3258 UniGene entries
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Transcripts per million
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EST count
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UniGene Entry
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21910
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259
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Hs.433455
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Enolase 1, (alpha)
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13281
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157
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Hs.169476
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Glyceraldehyde-3-phosphate dehydrogenase
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12604
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149
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Hs.498569
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Ribosomal protein S2
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12266
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145
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Hs.198281
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Pyruvate kinase, muscle
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12012
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142
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Hs.14376
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Actin, gamma 1
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9390
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111
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Hs.356572
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Ribosomal protein S3A
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8459
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100
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Hs.462864
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Annexin A2
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8290
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98
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Hs.387576
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Ribosomal protein S3
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7951
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94
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Hs.371889
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ATPase, Na+/K+ transporting, alpha 1 polypeptide
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7444
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88
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Hs.397609
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Ribosomal protein S16
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7359
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87
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Hs.439552
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Eukaryotic translation elongation factor 1 alpha 1
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Relative frequency based on tpm
Download UniGene Sequences
UNIX
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PC
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MAC
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