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Stratagene pancreas (#937208) (dbEST Library ID.493)

Organism: Homo sapiens
Tissue: Pancreas
Vector: pBluescript SK- (phagemid)
Lab host: SOLR cells (kanamycin resistant)
Cloning sites: EcoRI, XhoI

Library Description
Cloned unidirectionally. Primer: Oligo dT. Pancreatic adenocarcinoma cell line. Average insert size: 1.0 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

Sequence Submitters
Wilson RK
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108

References
Hillier LD, et al. Generation and analysis of 280,000 human expressed sequence tags. Genome Res 6, 807-828 (1996).

Distribution of member sequences in UniGene
5431 of the 6721 sequences from this library have been classified into 1814 UniGene entries

Transcripts per million   EST count UniGene Entry  
23380 127 Hs.132319 Ferritin, heavy polypeptide 1
20800 113 Hs.498569 Ribosomal protein S2
18960 103 Hs.433455 Enolase 1, (alpha)
14540 79 Hs.309517 Keratin 19
7360 40 Hs.76067 Heat shock 27kDa protein 1
6440 35 Hs.129673 Eukaryotic translation initiation factor 4A, isoform 1
6260 34 Hs.436441 Lamin A/C
6070 33 Hs.426930 Actin, beta
5330 29 Hs.296638 Growth differentiation factor 15
5330 29 Hs.2785 Keratin 17
4780 26 Hs.77961 Major histocompatibility complex, class I, B
   Relative frequency based on tpm


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