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Stratagene fibroblast (#937212) (dbEST Library ID.494)

Organism: Homo sapiens
Tissue: Skin
Vector: pBluescript SK- (phagemid)
Lab host: SOLR cells (kanamycin resistant)
Cloning sites: EcoRI, XhoI

Library Description
Cloned unidirectionally. Primer: Oligo dT. W138 cell line. Average insert size: 0.8 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'

Sequence Submitters
Wilson RK
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108

References
Hillier LD, et al. Generation and analysis of 280,000 human expressed sequence tags. Genome Res 6, 807-828 (1996).

Distribution of member sequences in UniGene
1243 of the 1640 sequences from this library have been classified into 562 UniGene entries

Transcripts per million   EST count UniGene Entry  
28150 35 Hs.435800 Vimentin
24930 31 Hs.132319 Ferritin, heavy polypeptide 1
12060 15 Hs.439552 Eukaryotic translation elongation factor 1 alpha 1
11260 14 Hs.8102 Ribosomal protein S20
10450 13 Hs.446628 Ribosomal protein S4, X-linked
10450 13 Hs.426930 Actin, beta
9650 12 Hs.421496 Transforming growth factor, beta-induced, 68kDa
8840 11 Hs.375919 Androgen-regulated protein
8840 11 Hs.374596 Tumor protein, translationally-controlled 1
8840 11 Hs.356572 Ribosomal protein S3A
8040 10 Hs.536798 Transcribed locus
   Relative frequency based on tpm


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