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Stratagene endothelial cell 937223 (dbEST Library ID.523)
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Organism:
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Homo sapiens
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Developmental stage:
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umbilical vein, 1 passage
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Tissue:
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Vascular
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Vector:
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pBluescript SK- (phagemid (ampicillin resistant))
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Lab host:
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SOLR (kanamycin resistant)
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Cloning sites:
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EcoRI, XhoI
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Library Description
Cloned unidirectionally. Primer: Oligo dT. Umbilical vein
endothelial cells, passaged once. Average insert size: 1.0 kb;
Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3'
~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'
Sequence Submitters
Wilson RK
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
References
 Hillier LD, et al. Generation and analysis of 280,000 human expressed sequence tags. Genome Res 6, 807-828 (1996).
Distribution of member sequences in UniGene
6366 of the 7171 sequences from this library have been classified into 1665 UniGene entries
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Transcripts per million
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EST count
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UniGene Entry
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17120
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109
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Hs.462864
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Annexin A2
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12250
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78
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Hs.443796
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Ribosomal protein, large, P0
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8320
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53
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Hs.449070
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Ribosomal protein L13a
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8320
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53
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Hs.439552
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Eukaryotic translation elongation factor 1 alpha 1
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8320
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53
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Hs.275865
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Ribosomal protein S18
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8160
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52
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Hs.75968
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Thymosin, beta 4, X-linked
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7850
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50
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Hs.381061
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Ribosomal protein L19
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7380
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47
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Hs.387576
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Ribosomal protein S3
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6910
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44
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Hs.83169
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Matrix metalloproteinase 1 (interstitial collagenase)
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6910
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44
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Hs.8102
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Ribosomal protein S20
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6910
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44
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Hs.356794
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Ribosomal protein S24
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Relative frequency based on tpm
Download UniGene Sequences
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