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NIH_MGC_16 (dbEST Library ID.5374)
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Organism:
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Homo sapiens
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Tissue:
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Eye
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Vector:
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pOTB7 (plasmid (chloramphenicol resistant, grown at 30C))
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Lab host:
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DH10B (phage-resistant)
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Cloning sites:
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XhoI, EcoRI
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Library Description
cDNA made by oligo-dT priming. Directionally cloned into
EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G).
Library constructed by Ling Hong in the laboratory of Gerald M.
Rubin (University of California, Berkeley) using ZAP-cDNA
synthesis kit (Stratagene) and Superscript II RT (Life
Technologies). Note: this is a NIH_MGC Library.
Sequence Submitters
Robert Strausberg, Ph.D.
Prange CK
Lawrence Livermore National Laboratory
Livermore, CA, USA
Ina Rolfs
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany
Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
Heubnerweg 6, D-14059 Berlin, Germany
Distribution of member sequences in UniGene
15032 of the 17255 sequences from this library have been classified into 3461 UniGene entries
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Transcripts per million
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EST count
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UniGene Entry
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51623
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776
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Hs.298280
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ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle
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15367
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231
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Hs.14376
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Actin, gamma 1
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13571
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204
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Hs.356572
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Ribosomal protein S3A
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11508
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173
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Hs.439552
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Eukaryotic translation elongation factor 1 alpha 1
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11508
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173
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Hs.119598
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Ribosomal protein L3
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10643
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160
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Hs.498569
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Ribosomal protein S2
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10510
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158
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Hs.256184
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Eukaryotic translation elongation factor 1 gamma
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9912
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149
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Hs.443796
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Ribosomal protein, large, P0
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9579
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144
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Hs.446608
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Tubulin, alpha, ubiquitous
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9047
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136
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Hs.169476
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Glyceraldehyde-3-phosphate dehydrogenase
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8182
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123
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Hs.356729
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Beta 5-tubulin
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Relative frequency based on tpm
Download UniGene Sequences
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