|
Lupski_sciatic_nerve (dbEST Library ID.7209)
|
Organism:
|
Homo sapiens
|
|
Developmental stage:
|
Adult
|
|
Sex:
|
male
|
|
Tissue:
|
Peripheral_Nervous_System
|
|
Vector:
|
pCMV-SPORT6 (Life Technologies) (plasmid (ampicillin resistant, 200 ug/ml))
|
|
Lab host:
|
DH10B
|
|
Cloning sites:
|
NotI, SalI
|
Library Description
cDNA made by oligo-dT priming. Directionally cloned using the
following adaptors: 5'-TCGACCCACGCGTCCG-3' and
5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCT(15)-3'. Size selected > 1 kb
for average insert length 1.87 kb. This is a primary library,
non-amplified. Library constructed by Life Technologies and
donated by J. Lupski, M.D./Ph.D. (Baylor College of Medicine)
and is available through Life Technologies.
Sequence Submitters
Robert Strausberg, Ph.D.
Distribution of member sequences in UniGene
5265 of the 6602 sequences from this library have been classified into 3538 UniGene entries
|
Transcripts per million
|
|
EST count
|
UniGene Entry
|
|
|
5120
|
|
27
|
Hs.171995
|
Kallikrein 3, (prostate specific antigen)
|
|
4930
|
|
26
|
Hs.169476
|
Glyceraldehyde-3-phosphate dehydrogenase
|
|
4550
|
|
24
|
Hs.406283
|
Homo sapiens, clone IMAGE:6069899, mRNA
|
|
3790
|
|
20
|
Hs.439552
|
Eukaryotic translation elongation factor 1 alpha 1
|
|
3600
|
|
19
|
Hs.181350
|
Kallikrein 2, prostatic
|
|
3410
|
|
18
|
Hs.14376
|
Actin, gamma 1
|
|
3220
|
|
17
|
Hs.183704
|
Ubiquitin C
|
|
2840
|
|
15
|
Hs.435953
|
Spondin 2, extracellular matrix protein
|
|
2650
|
|
14
|
Hs.279604
|
Desmin
|
|
2650
|
|
14
|
Hs.179526
|
Thioredoxin interacting protein
|
|
2460
|
|
13
|
Hs.119598
|
Ribosomal protein L3
|
Relative frequency based on tpm
Download UniGene Sequences
UNIX
-
PC
-
MAC
| 
