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NIH_MGC_42 (dbEST Library ID.7245)
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Organism:
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Homo sapiens
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Tissue:
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Pancreas
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Vector:
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pOTB7 (plasmid (chloramphenicol resistant, grown at 30C))
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Lab host:
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DH10B (phage-resistant)
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Cloning sites:
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XhoI, EcoRI
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Library Description
cDNA made by oligo-dT priming. Directionally cloned into
EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G).
Size-selected >500bp for average insert size 1.8kb. Library
constructed by Ling Hong in the laboratory of Gerald M. Rubin
(University of California, Berkeley) using ZAP-cDNA synthesis
kit (Stratagene) and Superscript II RT (Life Technologies).
Note: this is a NIH_MGC Library. |
Sequence Submitters
Robert Strausberg, Ph.D.
Ina Rolfs
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany
Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
Heubnerweg 6, D-14059 Berlin, Germany
Distribution of member sequences in UniGene
9666 of the 10751 sequences from this library have been classified into 2862 UniGene entries
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Transcripts per million
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EST count
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UniGene Entry
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20270
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196
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Hs.397609
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Ribosomal protein S16
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15930
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154
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Hs.14376
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Actin, gamma 1
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9410
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91
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Hs.356123
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Keratin 8
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8170
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79
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Hs.169476
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Glyceraldehyde-3-phosphate dehydrogenase
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7440
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72
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Hs.498569
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Ribosomal protein S2
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7240
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70
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Hs.406013
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Keratin 18
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6930
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67
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Hs.406455
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Prosaposin (variant Gaucher disease and variant metachromatic leukodystrophy)
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6200
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60
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Hs.433455
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Enolase 1, (alpha)
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6200
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60
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Hs.426930
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Actin, beta
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5690
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55
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Hs.99853
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Fibrillarin
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5060
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49
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Hs.309517
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Keratin 19
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Relative frequency based on tpm
Download UniGene Sequences
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