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NIH_MGC_97 (dbEST Library ID.8571)

Organism: Homo sapiens
Tissue: Testis
Vector: pBluescriptR (modified pBluescript KS+) (phagemid (ampicillin resistant))
Lab host: DH10B
Cloning sites: BamHI, SalI-XhoI (gtcgag)

Library Description
Oligo-dT primed using primer 5'-TTTTTTTTTTTTTTTTVN-3', size-selected for average insert size 2.2 kb and normalized to ROT 5. This is a primary library enriched for full-length clones and constructed using the Cap-trapper method (Carninci, in preparation). Library constructed by M. Brownstein (NIMH/NHGRI, National Institutes of Health). Note: this is a NIH_MGC Library.

Sequence Submitters
Robert Strausberg, Ph.D.

Scherer, S.W.; Rommens, J.M.; Tsui, L.C.; Vincent, J.B
Department of Genetics, The Hospital for Sick Children
555 University Avenue, Toronto, Ontario M5G 1X8, Canada

Prange CK
Lawrence Livermore National Laboratory
Livermore, CA, USA

Ina Rolfs
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany

Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
Heubnerweg 6, D-14059 Berlin, Germany

Distribution of member sequences in UniGene
18659 of the 21470 sequences from this library have been classified into 7240 UniGene entries

Transcripts per million   EST count UniGene Entry  
2304 43 Hs.435371 T-complex 11 (mouse)
2250 42 Hs.432648 Heat shock 70kDa protein 2
2143 40 Hs.446579 Heat shock 90kDa protein 1, alpha
1929 36 Hs.371820 Ankyrin repeat domain 7
1822 34 Hs.355983 Basic leucine zipper and W2 domains 1
1822 34 Hs.310960 Hypothetical protein FLJ21839
1714 32 Hs.372719 PHD finger protein 7
1607 30 Hs.177926 Exonuclease NEF-sp
1554 29 Hs.407922 Regulatory factor X, 4 (influences HLA class II expression)
1500 28 Hs.21331 Zwilch
1447 27 Hs.174083 Chromosome 21 open reading frame 13
   Relative frequency based on tpm


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