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NIH_MGC_41 (dbEST Library ID.8775)
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Organism:
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Homo sapiens
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Tissue:
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Skin
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Vector:
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pOTB7 (plasmid (chloramphenicol resistant, grown at 30C))
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Lab host:
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DH10B (phage-resistant)
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Cloning sites:
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XhoI, EcoRI
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Library Description
cDNA made by oligo-dT priming. Directionally cloned into
EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G).
Library constructed by Ling Hong in the laboratory of Gerald M.
Rubin (University of California, Berkeley) using ZAP-cDNA
synthesis kit (Stratagene) and Superscript II RT (Life
Technologies). Note: this is a NIH_MGC Library.
Sequence Submitters
Robert Strausberg, Ph.D.
Prange CK
Lawrence Livermore National Laboratory
Livermore, CA, USA
Ina Rolfs
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany
Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
Heubnerweg 6, D-14059 Berlin, Germany
Distribution of member sequences in UniGene
14546 of the 15936 sequences from this library have been classified into 2511 UniGene entries
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Transcripts per million
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EST count
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UniGene Entry
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22411
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326
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Hs.169476
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Glyceraldehyde-3-phosphate dehydrogenase
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17255
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251
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Hs.14376
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Actin, gamma 1
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9005
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131
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Hs.178551
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Ribosomal protein L8
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8387
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122
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Hs.57301
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High mobility group AT-hook 1
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8318
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121
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Hs.433427
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Ribosomal protein S17
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7562
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110
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Hs.498569
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Ribosomal protein S2
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6599
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96
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Hs.387576
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Ribosomal protein S3
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6462
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94
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Hs.119598
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Ribosomal protein L3
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6393
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93
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Hs.408073
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Ribosomal protein S6
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6324
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92
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Hs.433455
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Enolase 1, (alpha)
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6324
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92
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Hs.426930
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Actin, beta
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Relative frequency based on tpm
Download UniGene Sequences
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