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NCI_CGAP_Co3 (dbEST Library ID.882)
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Organism:
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Homo sapiens
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Sex:
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pooled
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Tissue:
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Colon
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Vector:
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pT7T3D-Pac (Pharmacia) with a modified polylinker (phagemid (ampicillin resistant))
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Lab host:
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DH10B
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Cloning sites:
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Not I, Eco RI
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Library Description
1st strand cDNA was prepared from 12 pooled bulk tumor samples
and primed with a Not I - oligo(dT) primer. Double-stranded
cDNA was ligated to Eco RI adaptors (Pharmacia), digested with
Not I and cloned into the Not I and Eco RI sites of the
modified pT7T3 vector. Library went through one round of
normalization.
Sequence Submitters
Robert Strausberg, Ph.D.
Ina Rolfs
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany
Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
Heubnerweg 6, D-14059 Berlin, Germany
Distribution of member sequences in UniGene
11699 of the 13591 sequences from this library have been classified into 6140 UniGene entries
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Transcripts per million
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EST count
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UniGene Entry
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4359
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51
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Hs.356342
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Hypothetical protein MGC10850
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2991
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35
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Hs.433670
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Ferritin, light polypeptide
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1795
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21
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Hs.407995
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Macrophage migration inhibitory factor (glycosylation-inhibiting factor)
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1453
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17
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Hs.75307
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H1 histone family, member X
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1453
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17
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Hs.410497
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Brain protein I3
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1453
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17
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Hs.381184
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Ribosomal protein S19
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1367
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16
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Hs.296638
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Growth differentiation factor 15
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1025
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12
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Hs.78344
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Myosin, heavy polypeptide 11, smooth muscle
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1025
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12
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Hs.150101
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Lysosomal-associated membrane protein 1
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940
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11
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Hs.25640
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Claudin 3
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940
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11
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Hs.163867
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NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 2, 8kDa
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Relative frequency based on tpm
Download UniGene Sequences
UNIX
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PC
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MAC
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