Biomolecular NMR at CARB
 
Detailed knowledge of the structure and dynamics of macromolecules is critical for a complete understanding of biochemical reaction mechanisms and molecular recognition processes. Such an understanding can impact biotechnology and bioengineering efforts in the areas of pharmaceutical development and biomaterials design.  Several research programs, involving nuclear magnetic resonance (NMR) spectroscopy, are under way at CARB in which scientists are developing and utilizing state-of-the art measurement methods, models and databases to study macromolecular structure, dynamics and interactions.  Research projects include investigations into the structure and dynamics of RNA and RNA-protein complexes involved in gene regulation and retroviral infection, proteins involved in Ca+2 -dependent signal transduction, G-protein coupled receptors, and structural genomics.  Studies are also underway which utilize NMR measurements to study macromolecular folding and stability.

CARB provides a vibrant atmosphere for conducting research in NMR spectroscopy. It maintains a state-of-the-art NMR facility that includes super-conducting magnets operating at 500 MHz and 600 MHz proton resonance frequencies, as well as a complement of resources for research in molecular biology, x-ray crystallography, fluorescence spectroscopy, fast kinetics, calorimetry and computational science.

Another high field spectrometer operating at proton resonance frequency of 600 MHz was installed in 2003. It has an ultrashield magnet with a cryoprobe that helps facilitate the solution studies of biological macromolecules including proteins, nucleic acids.

CARB is also housing a superconducting NMR magnet operating at 300 MHz for proton spectra. This system is equipped with a multinuclear broadband inverse probe, a Quad probe capable of observing 1H, 19F, 31P and 13C as well as the other probes. This particular spectrometer is used as a walk on instrument, and it is available for outside users. Contact our NMR Facility Manager, Dr. Nese Sari, to arrange spectrometer access.

2004 Biomolecular NMR Symposium at CARB

Facility manager

Dr. Nese Sari

9600 Gudelsky Drive, MD 20850
Voice:(301) 738-6284
NMR-Lab: (301) 738-6132

Groups with an Interest in NMR

Ames Lab
Email: james@carb.nist.gov
Phone: (301) 738-6120

Marino Lab
Email: marino@carb.nist.gov
Phone: (301) 738-6160

Orban Lab
Email: orban@carb.nist.gov
Phone: (301) 738-6221
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NMR-time Scheduling

CARB-NMR Group Meetings

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Updated: 3/19/2004

Questions or Problems