Technical Abstract: There is concern that gins located in boll weevil (Anthonomus grandis Bohemon) eradication zones may become points of reintroduction when they serve customers in a neighboring infested area. Previous work showed that transport of live weevils to the gin within cotton modules is common. We tested boll weevil survival through two typical machine sequences used in commercial cotton gins to clean and dry the seed cotton in advance of the gin stand, as well as through two inclined cylinder cleaners, and one or two tower dryers operated at different temperatures. Large numbers of laboratory-reared boll weevils were marked with fluorescent powder, fed into the test system, and recovered under black lights. We found no evidence of survival through the seed cotton cleaning systems or through the two inclined cleaners alone, even when the dryers were not heated. 95% upper confidence limits were calculated for the observed zero recoveries based on sample size and the binomial distribution, and these represent the statistical worst-case survival potential. Survival through heated tower dryers declined rapidly to zero at higher temperatures, especially when two dryers were running. Although we conclude that the potential for survival of weevils in the seed cotton to the gin stand is zero or close to zero, a small percentage of live weevils were recovered in the green boll/rock trap, which may represent the greatest threat of reintroduction at the gin. Escape of live weevils with the gin trash is also possible, and studies addressing this issue will be presented elsewhere.