Technical Abstract: A dairy goat operation in Minnesota experienced a sudden, markedly increased mortality among a subgroup of its neonatal goats (approximately 46%). The animals had diarrhea and dyspnea of one to two days duration prior to death. Necropsy of four goat kids revealed marked, acute, catarrhal enteritis and fibrinous pleuropneumonia. Basophilic inclusion bodies filling the entire nucleus were present in few enterocytes of the ileum of three goats. Adenovirus particles were detected in the pooled feces by electron microscopy and were subsequently isolated from the intestinal content together with a parvo-like virus (dependovirus). Morphology, physicochemical characteristics, and neutralization tests indicated that the adenovirus resembled ovine adenovirus-2 (OAdV-2). However, the restriction endonuclease pattern produced by the goat adenovirus was distinct from that of OAdV-2.