ARS | Publication request: Endometrial Cxc Chemokine and Conceptus Integrin Expression During Peri-Implantation Period in the Ewe

Submitted to: Biology Of Reproduction Abstracts
Publication Acceptance Date: May 14, 2004
Publication Date: August 20, 2004
Publisher's URL: http://www.ssr.org/Documents/BIRE_2004abstracts_93_282.pdf
Citation: Imakawa, K., Imai, M., Isuzugawa, K., Qin, J., Yamamoto, S., Takahashi, Y., Christenson, R.K. 2004. Endometrial Cxc Chemokine And Conceptus Integrin Expression During Peri-Implantation Period In The Ewe [abstract]. Biology Of Reproduction. 70 (Supplement):210-211. (Abstract #514)

Technical Abstract: For a pregnancy to be established, initial apposition and adhesion of the blastocyst to maternal endometrium must occur in a coordinated manner; however, a key factor(s) that mediate the ovine trophoblast cell attachment to the apical surface of the endometrium has not been identified. Previously, a CXC chemokine, interferon-gamma inducible protein 10 kDa (IP-10) localized in the endometrium was found stimulated by conceptus interferon-tau (IFNt), which in turn regulated conceptus integrin expressions. In this study, the expression of all 26 integrin subunits on the trophoblast was screened and the effects of other members of CXC chemokines, MIG and ITAC, alone or together with IP-10, were examined for the integrin subunit induction and adhesiveness of trophoblasts to the maternal endometrium. Effects of each CXC chemokine on the induction of conceptus integrins differed. These integrin heterodimers were further evaluated through the use of a trophoblast cell line, HTS-1 and found that integrins induced with CXC chemokines were alpha-5-beta-1, alpha-9-beta-1 and alpha-11-beta-1. Binding partners for each integrin subunits were extra cellular matrics (ECMs) such as osteopontin, fibronectin, fibronectin 120K, vitronectin and collagen type I and type IV. Thus, all of these ECMs were then evaluated for their adhesion activity and found that collagen type I, collagen type IV and fibronectin were major ECM molecules interacted with trophoblast cells. These observations indicate that all CXC chemokines appear to be functioning through a receptor, CXCR3, resulting in the increase in specific integrin expressions.